PMID- 20607705
OWN - NLM
STAT- MEDLINE
DCOM- 20101025
LR  - 20220408
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 49
IP  - 9
DP  - 2010 Sep
TI  - C11orf95-MKL2 is the resulting fusion oncogene of t(11;16)(q13;p13) in chondroid 
      lipoma.
PG  - 810-8
LID - 10.1002/gcc.20788 [doi]
AB  - Chondroid lipoma, a rare benign adipose tissue tumor, may histologically resemble 
      myxoid liposarcoma or extraskeletal myxoid chondrosarcoma, but is genetically 
      distinct. In this study, an identical reciprocal translocation, 
      t(11;16)(q13;p13), was identified in three chondroid lipomas, a finding 
      consistent with previously isolated reports. A fluorescence in situ hybridization 
      (FISH)-based positional cloning strategy using a series of bacterial artificial 
      chromosome (BAC) probe combinations designed to narrow the 16p13 breakpoint 
      revealed MKL2 as the candidate gene. Subsequent 5' RACE studies demonstrated 
      C11orf95 as the MKL2 fusion gene partner. MKL/myocardin-like 2 (MKL2) encodes 
      myocardin-related transcription factor B in a megakaryoblastic leukemia gene 
      family, and C11orf95 (chromosome 11 open reading frame 95) is a hypothetical 
      protein. Sequencing analysis of reverse transcription-polymerse chain reaction 
      (RT-PCR) generated transcripts from all three chondroid lipomas defined the 
      fusion as occurring between exons 5 and 9 of C11orf95 and MKL2, respectively. 
      Dual-color breakpoint spanning probe sets custom-designed for recognition of the 
      translocation event in interphase cells confirmed the anticipated rearrangements 
      of the C11orf95 and MKL2 loci in all cases. The FISH and RT-PCR assays developed 
      in this study can serve as diagnostic adjuncts for the identification of this 
      novel C11orf95-MKL2 fusion oncogene in chondroid lipoma.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Huang, Dali
AU  - Huang D
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha, NE 68198-3135, USA.
FAU - Sumegi, Janos
AU  - Sumegi J
FAU - Dal Cin, Paola
AU  - Dal Cin P
FAU - Reith, John D
AU  - Reith JD
FAU - Yasuda, Taketoshi
AU  - Yasuda T
FAU - Nelson, Marilu
AU  - Nelson M
FAU - Muirhead, David
AU  - Muirhead D
FAU - Bridge, Julia A
AU  - Bridge JA
LA  - eng
GR  - P30 CA036727-24S59009/CA/NCI NIH HHS/United States
GR  - 5 P30 CA036727-2452/CA/NCI NIH HHS/United States
GR  - U-10-CA98543-091/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (MRTFB protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lipoma/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oncogene Fusion/*genetics
MH  - Open Reading Frames
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/*genetics
MH  - Translocation, Genetic/*genetics
MH  - Young Adult
PMC - PMC2904421
MID - NIHMS203810
EDAT- 2010/07/08 06:00
MHDA- 2010/10/26 06:00
PMCR- 2011/09/01
CRDT- 2010/07/08 06:00
PHST- 2010/07/08 06:00 [entrez]
PHST- 2010/07/08 06:00 [pubmed]
PHST- 2010/10/26 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - 10.1002/gcc.20788 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2010 Sep;49(9):810-8. doi: 10.1002/gcc.20788.