PMID- 2060863
OWN - NLM
STAT- MEDLINE
DCOM- 19910802
LR  - 20191029
IS  - 8755-0199 (Print)
IS  - 8755-0199 (Linking)
VI  - 14
IP  - 3
DP  - 1991
TI  - Oxidative changes in hypoxic-reoxygenated rabbit heart: a consequence of hypoxia 
      rather than reoxygenation.
PG  - 179-85
AB  - Tissue changes consistent with oxidative damage in hypoxic/reoxygenated heart 
      tissue have not been well documented. We recently reported that oxidative 
      perturbations were evident in isolated-perfused rat heart tissue subjected to as 
      little as 10 min hypoxia and that these changes were not exacerbated by 
      reoxygenation. The mechanism and species specificity of this finding is not 
      known. Rabbit hearts, which lack measurable xanthine oxidase activity, were 
      examined for evidence of hypoxia-induced injury. The release of lactate 
      dehydrogenase into the coronary effluent gradually increased during the 
      retrograde perfusion of isolated rabbit hearts with hypoxic medium (containing 10 
      mM glucose and 2.5 mM calcium), and was slightly enhanced upon reoxygenation 
      after 60 min hypoxia. Cardiac glutathione content decreased significantly while 
      glutathione disulfide, protein-glutathione mixed disulfides, thiobaribturic acid 
      reactive substances (TBARS), and protein carbonyl contents increased 
      significantly after 60 min of hypoxia, compared to oxygenated controls. These 
      values were unaltered after 4 min of reoxygenation except for a loss of TBARS. 
      The oxidative changes observed in hypoxic rabbit hearts may be caused by energy 
      deficiency impairing normal reductive processes or by the generation of reactive 
      oxygen species as a result of abnormal cell functions, but cannot be related to 
      xanthine oxidase activity.
FAU - Park, Y
AU  - Park Y
AD  - Division of Pharmacology and Toxicology, College of Pharmacy, University of 
      Texas, Austin 78712-1074.
FAU - Kehrer, J P
AU  - Kehrer JP
LA  - eng
GR  - HL40695/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Free Radic Res Commun
JT  - Free radical research communications
JID - 8709453
RN  - 0 (Free Radicals)
RN  - GAN16C9B8O (Glutathione)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Coronary Disease/metabolism
MH  - Free Radicals
MH  - Glutathione/metabolism
MH  - Hypoxia/*metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Myocardial Reperfusion Injury/metabolism
MH  - Myocardium/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen/*metabolism
MH  - Rabbits
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.3109/10715769109088947 [doi]
PST - ppublish
SO  - Free Radic Res Commun. 1991;14(3):179-85. doi: 10.3109/10715769109088947.