PMID- 20608935
OWN - NLM
STAT- MEDLINE
DCOM- 20110209
LR  - 20210103
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Linking)
VI  - 101
IP  - 9
DP  - 2010 Sep
TI  - Protein overexpression and gene amplification of epidermal growth factor receptor 
      in adult testicular germ cell tumors: potential role in tumor progression.
PG  - 1970-6
LID - 10.1111/j.1349-7006.2010.01638.x [doi]
AB  - Little is known about the pathologic significance of epidermal growth factor 
      receptor (EGFR) expression in malignant testicular germ cell tumors (TGCTs) in 
      adults. From the primary tumor sites of a cohort of 110 TGCT cases, we obtained 
      209 histologically distinct components: 53 intratubular germ cell neoplasia 
      unclassified (IGCNU) lesions, 83 seminomas (66 pure-form seminomas and 17 
      seminoma components in the mixed-form with nonseminomatous TGCTs), 27 embryonal 
      carcinomas, eight choriocarcinomas, 18 yolk sac tumors, and 20 immature 
      teratomas. Samples were analyzed for expression of EGFR protein and EGFR gene 
      amplification by immunohistochemistry and fluorescence in situ hybridization 
      (FISH), respectively. Overexpression of the EGFR protein was detected in 28% of 
      seminomas (27% in the pure-form and 29% in the mixed-form), 11% of embryonal 
      carcinomas, 88% of choriocarcinomas, 44% of yolk sac tumors, and none of the 
      IGCNU lesions or immature teratomas. A higher copy number (>/=4 copies per cell) 
      and amplification of the EGFR gene were detected in 20% and 10% of seminomas, 13% 
      and 0% of embryonal carcinomas, 71% and 60% of choriocarcinomas, 15% and 8% of 
      yolk sac tumors, and none of the IGCNU lesions or immature teratomas, 
      respectively. Both higher copy number and amplification of the EGFR gene were 
      positively correlated with immunohistochemical overexpression of EGFR protein 
      (each P < 0.0001). These results suggest that overexpression of EGFR protein and 
      increased copy number or amplification of the EGFR gene occur relatively 
      frequently in primary TGCTs, and may play roles in the formation of invasive 
      cancer and in the progression, especially morphological evolution, of tumors.
CI  - (c) 2010 Japanese Cancer Association.
FAU - Miyai, Kosuke
AU  - Miyai K
AD  - Department of Basic Pathology, Tokorozawa, Saitama, Japan.
FAU - Yamamoto, Sohei
AU  - Yamamoto S
FAU - Asano, Tomohiko
AU  - Asano T
FAU - Tamai, Seiichi
AU  - Tamai S
FAU - Matsubara, Osamu
AU  - Matsubara O
FAU - Tsuda, Hitoshi
AU  - Tsuda H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chi-Square Distribution
MH  - Disease Progression
MH  - ErbB Receptors/*genetics/metabolism
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Neoplasms, Germ Cell and Embryonal/*genetics/metabolism/pathology
MH  - Testicular Neoplasms/*genetics/metabolism/pathology
MH  - Tissue Array Analysis
MH  - Tumor Burden
MH  - Young Adult
EDAT- 2010/07/09 06:00
MHDA- 2011/02/10 06:00
CRDT- 2010/07/09 06:00
PHST- 2010/07/09 06:00 [entrez]
PHST- 2010/07/09 06:00 [pubmed]
PHST- 2011/02/10 06:00 [medline]
AID - CAS1638 [pii]
AID - 10.1111/j.1349-7006.2010.01638.x [doi]
PST - ppublish
SO  - Cancer Sci. 2010 Sep;101(9):1970-6. doi: 10.1111/j.1349-7006.2010.01638.x.