PMID- 20616766
OWN - NLM
STAT- MEDLINE
DCOM- 20101027
LR  - 20211028
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 90
IP  - 5
DP  - 2010 Sep 15
TI  - Chronic graft versus host disease is associated with an immune response to 
      autologous human leukocyte antigen-derived peptides.
PG  - 555-63
LID - 10.1097/TP.0b013e3181e86b58 [doi]
AB  - BACKGROUND: Chronic graft-versus-host disease (cGVHD) is a major complication of 
      allogeneic bone marrow transplantation (BMT) the immunopathogenesis of which is 
      not well understood. Humoral and cellular immunity are both implicated, patients 
      express a range of autoantibodies, but the targets of cellular immunity are not 
      well defined. Autologous human leukocyte antigen (HLA)-derived peptides 
      constitute a significant proportion of the repertoire. METHODS: We have 
      investigated the response to HLA-derived peptides after allogeneic BMT using 
      gamma-interferon enzyme-linked immunospot assay (ELISPOT). We also studied the 
      release of this gamma-interferon by flow cytometry in a subgroup of responsive 
      patients. RESULTS: The peripheral blood mononuclear cell response was assessed by 
      gamma-interferon ELISPOT in 42 BMT recipients (21 with cGVHD) and 30 healthy 
      donors. Thirteen of 21 patients diagnosed with cGVHD responded to at least one 
      HLA-derived peptide compared with 1 of 21 patients without cGVHD (62% vs. 5%, 
      P<10) and 1 of 30 healthy donors. In all but one patient these peptides 
      correspond with the sequences of autologous HLA. The median single 
      peptide-specific response in ELISPOT was 43/10 peripheral blood mononuclear 
      cells. In a subgroup studied by flow cytometry, gamma-interferon production to 
      individual peptides occurred in 0.04% to 0.18% of CD4 T lymphocytes. CONCLUSION: 
      These observations identify HLA-derived peptides as targets of a cellular immune 
      response in cGVHD.
FAU - Smith, Helen J
AU  - Smith HJ
AD  - Department of Renal Medicine, Queen Elizabeth Hospital Birmingham, UK & School of 
      Immunity, Infection and Inflammation, College of Medical and Dental Sciences, 
      University of Birmingham, Birmingham, United Kingdom.
FAU - Hanvesakul, Rajesh
AU  - Hanvesakul R
FAU - Morgan, Matthew D
AU  - Morgan MD
FAU - Bentall, Andrew
AU  - Bentall A
FAU - Briggs, David
AU  - Briggs D
FAU - Clark, Fiona
AU  - Clark F
FAU - Pratt, Guy
AU  - Pratt G
FAU - Moss, Paul
AU  - Moss P
FAU - Larche, Mark
AU  - Larche M
FAU - Ball, Simon
AU  - Ball S
LA  - eng
GR  - G9901249/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Bone Marrow Transplantation/*immunology
MH  - Female
MH  - Graft vs Host Disease/etiology/*immunology
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - Immunophenotyping/methods
MH  - Interferon-gamma/biosynthesis/blood
MH  - Leukemia/surgery
MH  - Lymphocytes/immunology
MH  - Male
MH  - Transplantation, Homologous/immunology
EDAT- 2010/07/10 06:00
MHDA- 2010/10/28 06:00
CRDT- 2010/07/10 06:00
PHST- 2010/07/10 06:00 [entrez]
PHST- 2010/07/10 06:00 [pubmed]
PHST- 2010/10/28 06:00 [medline]
AID - 10.1097/TP.0b013e3181e86b58 [doi]
PST - ppublish
SO  - Transplantation. 2010 Sep 15;90(5):555-63. doi: 10.1097/TP.0b013e3181e86b58.