PMID- 20617552
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20171116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 397
IP  - 3
DP  - 2010 Jul 2
TI  - A novel in vivo inducible dendritic cell ablation model in mice.
PG  - 559-63
AB  - Dendritic cells (DCs) are involved in T cell activation via their uptake and 
      presentation of antigens. In vivo function of DCs was analyzed using transgenic 
      mouse models that express diphtheria toxin receptor (DTR) or the diphtheria 
      toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, 
      CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, 
      the in vivo function of each subset of DCs remains to be elucidated. Here, we 
      describe a new inducible DC ablation model, in which DTR expression is induced 
      under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette 
      (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, 
      expressing Cre recombinase under control of the cytomegalovirus immediate early 
      enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in 
      which DTR was selectively expressed in CD11c+ cells (iDTRDelta mice). We 
      successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic 
      CD11c+ cells in vivo after DT treatment in iDTRDelta mice. This mouse strain will 
      be a useful tool for generating mice lacking a specific subset of DCs using a 
      transgenic mouse strain, in which the Cre gene is expressed by a DC 
      subset-specific promoter.
FAU - Okuyama, Megumi
AU  - Okuyama M
AD  - Laboratory of Immune Regulation, Department of Microbiology and Immunology, 
      Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
FAU - Kayama, Hisako
AU  - Kayama H
FAU - Atarashi, Koji
AU  - Atarashi K
FAU - Saiga, Hiroyuki
AU  - Saiga H
FAU - Kimura, Taishi
AU  - Kimura T
FAU - Waisman, Ari
AU  - Waisman A
FAU - Yamamoto, Masahiro
AU  - Yamamoto M
FAU - Takeda, Kiyoshi
AU  - Takeda K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CD11c Antigen)
RN  - 0 (Hbegf protein, mouse)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/genetics
MH  - Dendritic Cells/*immunology
MH  - Heparin-binding EGF-like Growth Factor
MH  - Integrases/genetics
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - *Models, Animal
MH  - Promoter Regions, Genetic
EDAT- 2010/07/10 06:00
MHDA- 2010/08/13 06:00
CRDT- 2010/07/10 06:00
PHST- 2010/07/10 06:00 [entrez]
PHST- 2010/07/10 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - S0006-291X(10)01087-9 [pii]
AID - 10.1016/j.bbrc.2010.05.157 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Jul 2;397(3):559-63. doi: 
      10.1016/j.bbrc.2010.05.157.