PMID- 20621192
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20181201
IS  - 1095-9130 (Electronic)
IS  - 1046-2023 (Linking)
VI  - 52
IP  - 4
DP  - 2010 Dec
TI  - Automated brightfield break-apart in situ hybridization (ba-ISH) application: ALK 
      and MALT1 genes as models.
PG  - 352-8
LID - 10.1016/j.ymeth.2010.07.005 [doi]
AB  - Cancer diagnosis can be a complex process, which takes consideration of 
      histopathological, clinical, immunophenotypic, and genetic features. Since 
      non-random chromosomal translocations are specifically involved in the 
      development of various cancers, the detection of these gene aberrations becomes 
      increasingly important. In recent years, break-apart (or split-signal) 
      fluorescence in situ hybridization (FISH) has emerged as an advantageous 
      technique to detect gene translocations on tissue sections. However, FISH assays 
      are technically challenging and require specialized fluorescence microscopes. 
      Furthermore, the FISH signal is not stable for long term archiving due to photo 
      bleaching. Our objective was to demonstrate the feasibility of brightfield 
      break-apart in situ hybridization (ba-ISH) for anaplastic lymphoma kinase (ALK) 
      and mucosa-associated lymphoid tissue translocation protein 1 (MALT1) genes as 
      models. ALK or MALT1 break-apart probes were labeled with digoxigenin (DIG) or 
      2,4-dinitrophenyl (DNP) on both sides of a known gene breakpoint region and the 
      hybridization sites were visualized with the combination of alkaline phosphatase 
      (AP)-based blue and red detection. Therefore, normal genes are detected as purple 
      dots by mixing blue and red colors while translocated genes are detected as 
      isolated blue or red dots. Formalin-fixed, paraffin-embedded tonsil was used as 
      control for the co-localized 5' and 3' probes. Gene translocations of ALK or 
      MALT1 were detected as separate blue and red dots on ALCL and MALT lymphoma 
      cases. Thus, ISH analyses of gene translocations can be conducted with a regular 
      light microscope and the long term archiving of break-apart ISH slides can be 
      achieved.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Nitta, Hiroaki
AU  - Nitta H
AD  - Office of Medical Affairs, Ventana Medical Systems, Inc., 1910 E Innovation Park 
      Drive, Tucson, AZ 85755, USA. hiro.nitta@ventana.roche.com
FAU - Zhang, Wenjun
AU  - Zhang W
FAU - Kelly, Brian D
AU  - Kelly BD
FAU - Miller, Melanie
AU  - Miller M
FAU - Pestic-Dragovich, Lidija
AU  - Pestic-Dragovich L
FAU - Bieniarz, Christopher
AU  - Bieniarz C
FAU - Vasicek, Thomas J
AU  - Vasicek TJ
FAU - Marafioti, Teresa
AU  - Marafioti T
FAU - Rimsza, Lisa
AU  - Rimsza L
FAU - Grogan, Thomas M
AU  - Grogan TM
LA  - eng
PT  - Journal Article
DEP - 20100716
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (DNA Probes)
RN  - 0 (Neoplasm Proteins)
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (MALT1 protein, human)
RN  - EC 3.4.22.- (Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein)
SB  - IM
MH  - Anaplastic Lymphoma Kinase
MH  - Caspases/*genetics
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Lymphoma, B-Cell, Marginal Zone/genetics
MH  - Lymphoma, Large-Cell, Anaplastic/genetics
MH  - Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
MH  - Neoplasm Proteins/*genetics
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Receptor Protein-Tyrosine Kinases
MH  - Translocation, Genetic/genetics
EDAT- 2010/07/14 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/07/13 06:00
PHST- 2010/06/25 00:00 [received]
PHST- 2010/07/05 00:00 [accepted]
PHST- 2010/07/13 06:00 [entrez]
PHST- 2010/07/14 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - S1046-2023(10)00186-6 [pii]
AID - 10.1016/j.ymeth.2010.07.005 [doi]
PST - ppublish
SO  - Methods. 2010 Dec;52(4):352-8. doi: 10.1016/j.ymeth.2010.07.005. Epub 2010 Jul 
      16.