PMID- 20623778 OWN - NLM STAT- MEDLINE DCOM- 20140422 LR - 20130813 IS - 1097-0029 (Electronic) IS - 1059-910X (Linking) VI - 74 IP - 3 DP - 2011 Mar TI - Influence of Er:YAG laser frequency on dentin caries removal capacity. PG - 281-6 LID - 10.1002/jemt.20902 [doi] AB - The purposes of this study were to evaluate in vitro the influence of different frequencies of Er:YAG laser on the human dentin caries removal capacity. Thirty fragments obtained from third molars were randomly assigned into three groups (n = 10) according to the laser frequency used: 4, 6, and 10 Hz. The caries lesion (+/-1 mm deep) was induced before the irradiation by S.mutans cultures for 6 weeks. The specimens of all groups were irradiated with 200 mJ of energy in noncontact and focused mode under constant refrigeration (water flow: 2.5 mL/min). Quantitative analysis of the caries removal was performed by DIAGNOdent and the Axion Vision software. Qualitative analysis was performed by Scanning electron microscope (SEM) and light microscope (LM). Data were analyzed by ANOVA and Fishers' tests. The DIAGNOdent revealed that the caries removal was similar with 4 and 6 Hz and was superior with 10 Hz (P < 0.05). The analysis with Axion Vision software revealed that the caries removal was similar with 6 and 10 Hz and the 4 Hz group promoted the lowest caries removal. Through SEM morphologic analysis, some specimens irradiated with 4 Hz presented, under the demineralized dentin, a disorganized collagenous matrix. The LM images revealed that all frequencies used promoted irregular caries removal, being observed over preparations with 6 and 10 Hz. It can be concluded that the increase of Er:YAG laser frequency provided a higher dentin caries removal without selectivity to the disorganized dentin. CI - Copyright (c) 2010 Wiley-Liss, Inc. FAU - Raucci-Neto, Walter AU - Raucci-Neto W AD - Department of Restorative Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. rgpalma@forp.usp.br, rauccineto@yahoo.com.br. FAU - Chinelatti, Michelle Alexandra AU - Chinelatti MA FAU - Ito, Izabel Yoko AU - Ito IY FAU - Pecora, Jesus Djalma AU - Pecora JD FAU - Palma-Dibb, Regina Guenka AU - Palma-Dibb RG LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Microsc Res Tech JT - Microscopy research and technique JID - 9203012 SB - IM MH - Dental Caries/*therapy MH - Dentin/*radiation effects MH - Humans MH - Lasers, Solid-State/*therapeutic use MH - Light MH - Molar, Third/radiation effects OTO - NOTNLM OT - Er:YAG laser OT - LM OT - SEM OT - caries removal EDAT- 2010/07/14 06:00 MHDA- 2014/04/23 06:00 CRDT- 2010/07/13 06:00 PHST- 2010/03/09 00:00 [received] PHST- 2010/06/06 00:00 [accepted] PHST- 2010/07/13 06:00 [entrez] PHST- 2010/07/14 06:00 [pubmed] PHST- 2014/04/23 06:00 [medline] AID - 10.1002/jemt.20902 [doi] PST - ppublish SO - Microsc Res Tech. 2011 Mar;74(3):281-6. doi: 10.1002/jemt.20902.