PMID- 20624074
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20100719
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Linking)
VI  - 26
IP  - 7
DP  - 2010 Jul
TI  - Genetic characterization of HIV type 1 among patients with suspected immune 
      reconstitution inflammatory syndrome after initiation of antiretroviral therapy 
      in Kenya.
PG  - 833-8
LID - 10.1089/aid.2009.0229 [doi]
AB  - Antiretroviral therapy (ART) has improved the survival of HIV patients but is 
      also associated with unique manifestations of disease in some subjects during the 
      initial months of therapy. Immune reconstitution inflammatory syndrome (IRIS) is 
      a disorder among individuals starting ART, with no evidence-based treatment and 
      management guidelines. We characterized HIV-1 and determined drug resistance 
      among 14 Kenyan patients with suspected IRIS after ART initiation in 2005. 
      Polymerase chain reaction, sequencing, and phylogenetic analysis of viral pol and 
      env showed the following HIV-1 subtypes: A1/A1/A1 (pol-RT/gp41/C2V3), 5; A1/C/A1, 
      1; A1/D/A1, 2; D/A1/A1, 1; D/C/A1, 1; D/D/A1, 2; D/D/D, 1; and D/A1/A2, 1. Three 
      patients had viruses with major drug resistance-associated mutations. These 
      included nucleoside reverse transcriptase inhibitor (RTI) mutations: M41L, K65R, 
      D67N, K70R, M184V, and K219Q, and nonnucleoside RTI mutations: K101P, L100I, 
      K103N, and Y181C. Twelve patients harbored viruses that are predicted to use 
      chemokine coreceptor 5 (CCR5) whereas two had variant viruses predicted to use 
      the CXCR4 coreceptor. Drug resistance may not be the only cause of ART adverse 
      events. HIV-1 characterization would be important before and during HIV therapy 
      to avoid treatment failure.
FAU - Lihana, Raphael W
AU  - Lihana RW
AD  - Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya. 
      Rlihana@kemri.org
FAU - Khamadi, Samoel A
AU  - Khamadi SA
FAU - Lubano, Kizito
AU  - Lubano K
FAU - Mwangi, Joseph
AU  - Mwangi J
FAU - Kinyua, Joyceline G
AU  - Kinyua JG
FAU - Okoth, Vincent O
AU  - Okoth VO
FAU - Lagat, Nancy J
AU  - Lagat NJ
FAU - Okoth, Fredrick A
AU  - Okoth FA
FAU - Songok, Elijah M
AU  - Songok EM
FAU - Makokha, Ernest P
AU  - Makokha EP
LA  - eng
SI  - GENBANK/FJ599374
SI  - GENBANK/FJ599375
SI  - GENBANK/FJ599376
SI  - GENBANK/FJ599377
SI  - GENBANK/FJ599378
SI  - GENBANK/FJ599381
SI  - GENBANK/FJ599382
SI  - GENBANK/FJ599383
SI  - GENBANK/FJ599384
SI  - GENBANK/FJ599385
SI  - GENBANK/FJ599386
SI  - GENBANK/FJ599387
SI  - GENBANK/FJ599388
SI  - GENBANK/FJ599389
SI  - GENBANK/FJ599390
SI  - GENBANK/FJ599391
SI  - GENBANK/FJ599392
SI  - GENBANK/FJ599393
SI  - GENBANK/FJ599394
SI  - GENBANK/FJ599395
SI  - GENBANK/FJ599396
SI  - GENBANK/FJ599397
SI  - GENBANK/FJ599398
SI  - GENBANK/FJ599399
SI  - GENBANK/FJ599482
SI  - GENBANK/FJ599483
SI  - GENBANK/FJ599484
SI  - GENBANK/FJ599485
SI  - GENBANK/FJ599486
SI  - GENBANK/FJ599487
SI  - GENBANK/FJ599488
SI  - GENBANK/FJ599489
SI  - GENBANK/FJ599490
SI  - GENBANK/FJ599491
SI  - GENBANK/FJ599492
SI  - GENBANK/FJ599493
SI  - GENBANK/FJ599494
SI  - GENBANK/FJ599495
SI  - GENBANK/FJ599496
SI  - GENBANK/FJ599497
SI  - GENBANK/FJ599498
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Anti-HIV Agents)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (pol Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Substitution/genetics
MH  - Anti-HIV Agents/adverse effects/*therapeutic use
MH  - Antiretroviral Therapy, Highly Active/*adverse effects
MH  - Drug Resistance, Viral
MH  - Female
MH  - HIV Infections/*complications/drug therapy/*virology
MH  - HIV-1/*classification/*genetics/isolation & purification
MH  - Humans
MH  - Immune Reconstitution Inflammatory Syndrome/*virology
MH  - Kenya
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Mutation, Missense
MH  - Sequence Analysis, DNA
MH  - env Gene Products, Human Immunodeficiency Virus/genetics
MH  - pol Gene Products, Human Immunodeficiency Virus/genetics
EDAT- 2010/07/14 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/07/14 06:00
PHST- 2010/07/14 06:00 [entrez]
PHST- 2010/07/14 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - 10.1089/aid.2009.0229 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2010 Jul;26(7):833-8. doi: 10.1089/aid.2009.0229.