PMID- 20625836
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20211020
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 21
IP  - 11
DP  - 2010 Nov
TI  - Thermoresponsive poly(N-vinylcaprolactam) cryogels: synthesis and its biophysical 
      evaluation for tissue engineering applications.
PG  - 2937-45
LID - 10.1007/s10856-010-4124-3 [doi]
AB  - The thermoresponsive poly(N-vinylcaprolactam) (PVCl) based cryogel network were 
      synthesized and characterized with respect to physical and biological properties. 
      The PVCl cryogel crosslinked with polyethylene glycol-diacrylate (PEGda) was 
      synthesized in 1% dimethyl sulfoxide containing aqueous medium at -12 degrees C for 12-14 
      h. The cryogel synthesized in this manner were highly spongy in nature and can 
      absorb water in its porous network. These polymeric cryogel networks have good 
      physical morphology as confirmed by scanning electron microscopy. The estimated 
      porosity of these cryogels was 90% as demonstrated by various methods based on 
      absorption of water and cyclohexane. The median pore diameter and surface area 
      was 30 mum and 2.0253 m(2)/g, respectively as confirmed by analysis on mercury 
      porosimeter. These materials can interact with biological system without any 
      cytotoxic effects. Change in temperature influenced the adsorption of fetal 
      bovine serum (FBS) on PVCl scaffold which showed maximum protein adsorption at 
      37 degrees C, as compared to that at 25 degrees C. Furthermore, the fibroblast cell adhesion 
      studies showed the potential of these PVCl based cryogels as tissue engineering 
      scaffolds.
FAU - Srivastava, Akshay
AU  - Srivastava A
AD  - Department of Biological Sciences and Bioengineering, Indian Institute of 
      Technology Kanpur, 208016 Kanpur, India.
FAU - Kumar, Ashok
AU  - Kumar A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100713
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cryogels)
RN  - 0 (Hydrogels)
RN  - 0 (Polymers)
RN  - 059QF0KO0R (Water)
RN  - 25189-83-7 (poly-N-vinylcaprolactam)
RN  - 6879X594Z8 (Caprolactam)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Biocompatible Materials/chemical synthesis/chemistry
MH  - Biophysical Phenomena
MH  - COS Cells
MH  - Caprolactam/adverse effects/*analogs & derivatives/chemistry/metabolism
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Chlorocebus aethiops
MH  - Cryogels
MH  - Hydrogels/adverse effects/*chemical synthesis/*chemistry/metabolism
MH  - Materials Testing/methods
MH  - Microscopy, Electron, Scanning
MH  - Polymers/adverse effects/*chemistry/metabolism
MH  - *Temperature
MH  - Tissue Engineering/*instrumentation/methods
MH  - Tissue Scaffolds/chemistry
MH  - Water/metabolism/pharmacology
EDAT- 2010/07/14 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/07/14 06:00
PHST- 2010/04/16 00:00 [received]
PHST- 2010/06/29 00:00 [accepted]
PHST- 2010/07/14 06:00 [entrez]
PHST- 2010/07/14 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - 10.1007/s10856-010-4124-3 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2010 Nov;21(11):2937-45. doi: 10.1007/s10856-010-4124-3. 
      Epub 2010 Jul 13.