PMID- 20625918
OWN - NLM
STAT- MEDLINE
DCOM- 20110429
LR  - 20211020
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 60
IP  - 1
DP  - 2011 Jan
TI  - IgE-dependent sensitization increases responsiveness to LPS but does not modify 
      development of endotoxin tolerance in mast cells.
PG  - 19-27
LID - 10.1007/s00011-010-0230-4 [doi]
AB  - OBJECTIVE: Effects of immunoglobulin E (IgE)-dependent sensitization on the 
      response to bacterial lipopolysaccharide (LPS) were analyzed in mast cells. 
      METHODS: Murine bone marrow-derived mast cells (BMMCs) were sensitized or not 
      with IgE before stimulation with LPS. TLR4 and co-receptors expression was 
      analyzed by flow cytometry and RT-PCR, TNF-alpha production by ELISA, IKK and IkappaB 
      activation by western blot or immunoprecipitation. NFkappaB nuclear translocation was 
      determined by EMSA. RESULTS: IgE-sensitized BMMCs secreted larger amounts of 
      TNF-alpha than non-sensitized cells shortly after LPS challenge. No change in TLR4, 
      CD14 or MD-2 expression was detected after the IgE-dependent sensitization 
      process, whereas TLR4-dependent phosphorylation of IKK and IkappaB was augmented. 
      IgE-dependent sensitization increased basal NFkappaB activity. Endotoxin tolerance 
      was not affected by the IgE-dependent sensitization process. CONCLUSIONS: 
      IgE-induced sensitization primes mast cells for higher response to LPS through 
      pre-activation of NFkappaB transcription factor. IgE-dependent sensitization does not 
      modify events leading to endotoxin tolerance.
FAU - Medina-Tamayo, Jaciel
AU  - Medina-Tamayo J
AD  - Departamento de Farmacobiologia, Centro de Investigacion y de Estudios Avanzados 
      del IPN, Sede Sur, Calzada de los Tenorios No 235, Col Granjas Coapa, Tlalpan, 
      Mexico, DF, Mexico.
FAU - Ibarra-Sanchez, Alfredo
AU  - Ibarra-Sanchez A
FAU - Padilla-Trejo, Alejandro
AU  - Padilla-Trejo A
FAU - Gonzalez-Espinosa, Claudia
AU  - Gonzalez-Espinosa C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100713
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (BAY 11-7085)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitriles)
RN  - 0 (Sulfones)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Bone Marrow Cells/cytology/immunology
MH  - Cells, Cultured
MH  - I-kappa B Kinase/metabolism
MH  - Immune Tolerance/*immunology
MH  - Immunoglobulin E/*immunology
MH  - Lipopolysaccharides/*immunology/pharmacology
MH  - Mast Cells/cytology/drug effects/*immunology
MH  - Mice
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Nitriles
MH  - Sulfones
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
EDAT- 2010/07/14 06:00
MHDA- 2011/04/30 06:00
CRDT- 2010/07/14 06:00
PHST- 2010/04/06 00:00 [received]
PHST- 2010/06/23 00:00 [accepted]
PHST- 2010/06/08 00:00 [revised]
PHST- 2010/07/14 06:00 [entrez]
PHST- 2010/07/14 06:00 [pubmed]
PHST- 2011/04/30 06:00 [medline]
AID - 10.1007/s00011-010-0230-4 [doi]
PST - ppublish
SO  - Inflamm Res. 2011 Jan;60(1):19-27. doi: 10.1007/s00011-010-0230-4. Epub 2010 Jul 
      13.