PMID- 20626058
OWN - NLM
STAT- MEDLINE
DCOM- 20110119
LR  - 20211203
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 38
IP  - 4
DP  - 2010
TI  - Gingko biloba extract (EGb 761) prevents cerebral ischemia-induced p70S6 kinase 
      and S6 phosphorylation.
PG  - 727-34
AB  - EGb 761 is an extract of Gingko biloba that exhibits neuroprotective effects 
      against cerebral ischemia. The mammalian target of rapamycin (mTOR) is a critical 
      downstream effector of Akt and a central regulator of ribosomal biogenesis and 
      protein synthesis. We investigated whether EGb 761 regulates Akt downstream 
      targets, including mTOR, p70S6 kinase, and S6 phosphorylation. Adult male rats 
      were treated with vehicle or EGb 761 (100 mg/kg) prior to middle cerebral artery 
      occlusion (MCAO). Brains were collected at 24 hours after MCAO and the cerebral 
      cortex regions were examined. We previously showed that EGb 761 significantly 
      reduces infarct volume and decreases the number of TUNEL-positive cells in the 
      cerebral cortex. Ischemic brain injury induces a decrease in Akt up-stream 
      target, PDK1 phosphorylation. The levels of phospho-mTOR, phospho-p70S6 kinase, 
      and phospho-S6 are subsequently decreased in regions affected by ischemic injury. 
      However, EGb 761 prevented injury-induced decreases in these protein levels. We 
      confirmed that EGb 761 inhibits injury-induced decreases in the number of 
      positive cells for phospho-p70S6 kinase and phospho-S6. The results of this study 
      provide evidence that EGb 761 protects neuronal cells against ischemic brain 
      injury by preventing injury-induced decreases in p70S6 kinase and S6 
      phosphorylation.
FAU - Koh, Phil-Ok
AU  - Koh PO
AD  - Department of Anatomy, Research Institute of Life Science, Gyeongsang National 
      University, Jinju, South Korea. pokoh@gsnu.ac.kr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Pdk1 protein, rat)
RN  - 0 (Plant Extracts)
RN  - 0 (Pyruvate Dehydrogenase Acetyl-Transferring Kinase)
RN  - 0 (Ribosomal Proteins)
RN  - 19FUJ2C58T (Ginkgo biloba extract)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/metabolism/*prevention & control
MH  - Cerebral Cortex/drug effects/metabolism
MH  - Cerebral Infarction/metabolism/*prevention & control
MH  - *Ginkgo biloba
MH  - In Situ Nick-End Labeling
MH  - Infarction, Middle Cerebral Artery
MH  - Male
MH  - Neuroprotective Agents/*pharmacology/therapeutic use
MH  - Phosphorylation
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Pyruvate Dehydrogenase Acetyl-Transferring Kinase
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - Ribosomal Proteins/*metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
EDAT- 2010/07/14 06:00
MHDA- 2011/01/20 06:00
CRDT- 2010/07/14 06:00
PHST- 2010/07/14 06:00 [entrez]
PHST- 2010/07/14 06:00 [pubmed]
PHST- 2011/01/20 06:00 [medline]
AID - S0192415X10008196 [pii]
AID - 10.1142/S0192415X10008196 [doi]
PST - ppublish
SO  - Am J Chin Med. 2010;38(4):727-34. doi: 10.1142/S0192415X10008196.