PMID- 20626178
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20100714
IS  - 0024-7758 (Print)
IS  - 0024-7758 (Linking)
VI  - 55
IP  - 5-6
DP  - 2010 May-Jun
TI  - Simultaneous detection of imprinted gene expression (p57(KIP2)) and molecular 
      cytogenetics (FICTION) in the evaluation of molar pregnancies.
PG  - 219-28
AB  - OBJECTIVE: To simultaneously evaluate the p57(KIP2) antibody expression and 
      genotype of individual cells from paraffin sections of molar pregnancies. STUDY 
      DESIGN: Paraffin sections from 10 typical and unusual molar pregnancies were 
      evaluated with the FICTION technique (Fluorescence Immunophenotyping and 
      Interphase Cytogenetics as a Tool for the Investigation of Neoplasms), using 
      immunofluorescence staining for the p57(KIP2) antibody and enumeration 
      fluorescence in situ hybridization (FISH) probes. The unusual cases included 
      androgenetic/ biparental chimeric complete hydatidiform moles (CHM) and mosaic 
      partial hydatidiform moles (PHM). The unusual molar conceptions provided insight 
      into interpreting atypical p57(KIP2) staining patterns and identifying 
      androgenetic cells. RESULTS: The androgenetic/biparental chimeric CHMs 
      demonstrated a negative p57(KIP2) Staining pattern for the androgenetic cells and 
      positive staining for the biparental cells. Concordantly, the FISH results showed 
      delineation between the androgenetic cells and the biparental cells, indicating 2 
      distinct genotypes. Also, in the 2 cases of mosaic PHM, the partial loss of 
      p57(KIP2) antibody staining was due to mosaic loss of chromosome 11, assumed to 
      be the maternal copy. This provides a biological explanation as to how false 
      interpretation could occur when evaluating p57(KIP2) immunostaining results. 
      CONCLUSION: The FICTION technique is a valuable ancillary tool for simultaneously 
      evaluating the genotype and p57(KIP2) expression in unusual molar pregnancies.
FAU - Hoffner, Lori
AU  - Hoffner L
AD  - Magee-Women's Research Institute, Pittsburgh, PA 15213, USA.
FAU - Parks, W Tony
AU  - Parks WT
FAU - Swerdlow, Steven H
AU  - Swerdlow SH
FAU - Carson, Jason C
AU  - Carson JC
FAU - Surti, Urvashi
AU  - Surti U
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Reprod Med
JT  - The Journal of reproductive medicine
JID - 0173343
RN  - 0 (Autoantibodies)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p57)
SB  - IM
MH  - Autoantibodies/*analysis/metabolism
MH  - Chimera/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p57/*genetics/immunology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Hydatidiform Mole/*genetics/pathology
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Mosaicism
MH  - Pregnancy
MH  - Uterine Neoplasms/*genetics/pathology
EDAT- 2010/07/16 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/07/15 06:00
PHST- 2010/07/15 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
PST - ppublish
SO  - J Reprod Med. 2010 May-Jun;55(5-6):219-28.