PMID- 20628248
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20100714
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 129
IP  - 1-3
DP  - 2010 Jul
TI  - Recent and frequent insertions of chloroplast DNA into maize nuclear chromosomes.
PG  - 17-23
LID - 10.1159/000312724 [doi]
AB  - Organellar DNA transfer into the nucleus and incorporation into chromosomes are 
      continuing processes. We have examined chloroplast DNA insertions in maize 
      chromosomes using fluorescence in situ hybridization (FISH). Probes to detect the 
      nuclear-plastid sequences (NUPTs) were generated using 14 overlapping fragments 
      of the chloroplast genome. Using current FISH methods, detection of NUPTs on 
      mitotic metaphase chromosomes requires relatively large insertions (>3 kb) with 
      strong sequence similarity to chloroplast DNA. In the B73 inbred line, 
      hybridization of each fragment separately resolved a total of 49 NUPT sites; 
      fewer sites (30) were detectable when all the probes were combined. The combined 
      set of probes was hybridized to 10 genetically useful maize inbred lines and 
      identified 19-30 NUPTs in each line. In all but two of the lines, a prominent 
      NUPT was present on the long arm of chromosome 5. Collectively, the number of 
      NUPTs exceeds the number of nuclear-mitochondrial (NUMT) sites identified within 
      the same set of inbred lines. A majority of the NUPTs are found at sites that are 
      different from the NUMTs. Like NUMTs, the positions of the NUPTs vary greatly 
      among the lines, suggesting that the transfers are recent as well as frequent. 
      Thus, insertions of large segments of chloroplast and mitochondrial DNA are 
      components of the dynamic fraction of maize nuclear genomes.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Roark, L M
AU  - Roark LM
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
FAU - Hui, A Y
AU  - Hui AY
FAU - Donnelly, L
AU  - Donnelly L
FAU - Birchler, J A
AU  - Birchler JA
FAU - Newton, K J
AU  - Newton KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100713
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Primers)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Chloroplast)
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Sequence
MH  - Cell Nucleus/genetics
MH  - Chromosomes, Plant/*genetics
MH  - DNA Primers/genetics
MH  - DNA Transposable Elements
MH  - DNA, Chloroplast/*genetics
MH  - DNA, Mitochondrial/genetics
MH  - DNA, Plant/genetics
MH  - Evolution, Molecular
MH  - Genome, Chloroplast
MH  - Genome, Mitochondrial
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Inbreeding
MH  - Species Specificity
MH  - Time Factors
MH  - Zea mays/classification/*genetics
EDAT- 2010/07/16 06:00
MHDA- 2010/08/07 06:00
CRDT- 2010/07/15 06:00
PHST- 2010/07/15 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 000312724 [pii]
AID - 10.1159/000312724 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2010 Jul;129(1-3):17-23. doi: 10.1159/000312724. Epub 2010 
      Jul 13.