PMID- 20628251
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20100714
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 129
IP  - 1-3
DP  - 2010 Jul
TI  - Integration of genetic, physical, and cytogenetic maps for Brassica rapa 
      chromosome A7.
PG  - 190-8
LID - 10.1159/000314640 [doi]
AB  - Bacterial artificial chromosome (BAC) contigs have been genetically mapped to the 
      10 linkage groups of Brassica rapa by BAC end sequences (BES). To integrate the 
      genetic, physical, and cytogenetic maps, fluorescence in situ hybridization 
      (FISH) was used to anchor the assembly of BAC contigs onto Brassica chromosomes 
      using representative BACs. This BAC-FISH approach can be used to identify 
      chromosome arms on separate mitotic metaphase chromosomes or to map multiple BACs 
      to single long pachytene chromosomes. As part of an international consortium that 
      is sequencing the B. rapa genome, we integrated the linkage and physical maps 
      with the B. rapa cytogenetic map for chromosome A7 by hybridizing BACs to mitotic 
      chromosomes and along the length of pachytene chromosome spreads. A total of 31 
      BACs that were putatively located on A7 were used as probes for FISH analyses; 
      however, only 19 BACs mapped unambiguously to A7 while the remaining BACs either 
      mapped to other chromosomes or hybridized to multiple locations. We then created 
      a multicolor FISH cocktail of 16 BAC probes to simultaneously hybridize the 
      entire length of the A7 chromosome. We successfully applied the 16 A7 BAC probe 
      mix to B. rapa, B. oleracea, and domesticated and resynthesized genotypes of B. 
      napus to demonstrate that this approach can facilitate studies of genome 
      evolution by integrating the genetic, physical, and cytogenetic maps among 
      closely related species of Brassica.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Xiong, Z
AU  - Xiong Z
AD  - Division of Biological Sciences, Christopher S. Bond Life Sciences Center, 
      University of Missouri, Columbia, MO 65211, USA.
FAU - Kim, J S
AU  - Kim JS
FAU - Pires, J C
AU  - Pires JC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100713
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Brassica rapa/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Plant/*genetics
MH  - Contig Mapping
MH  - Cytogenetic Analysis/*methods
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Physical Chromosome Mapping
EDAT- 2010/07/16 06:00
MHDA- 2010/08/07 06:00
CRDT- 2010/07/15 06:00
PHST- 2010/07/15 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 000314640 [pii]
AID - 10.1159/000314640 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2010 Jul;129(1-3):190-8. doi: 10.1159/000314640. Epub 2010 
      Jul 13.