PMID- 20628842
OWN - NLM
STAT- MEDLINE
DCOM- 20120120
LR  - 20211020
IS  - 1476-3524 (Electronic)
IS  - 1029-8428 (Linking)
VI  - 19
IP  - 4
DP  - 2011 May
TI  - Effects of chlorinated acetates on the glutathione metabolism and on glycolysis 
      of cultured astrocytes.
PG  - 628-37
LID - 10.1007/s12640-010-9209-8 [doi]
AB  - The chlorinated acetates monochloroacetate (MCA), dichloroacetate (DCA), and 
      trichloroacetate (TCA) are generated in water disinfection processes and are 
      formed during metabolic detoxification of industrial solvents such as 
      trichloroethylene. In order to test for consequences of an exposure of brain 
      cells to the different chlorinated acetates, glutathione levels and lactate 
      production of primary astrocyte cultures were investigated as indicators for the 
      potential of chlorinated acetates to disturb cellular detoxification processes 
      and glucose metabolism, respectively. Application of MCA to cultured astrocytes 
      caused a time and concentration dependent deprivation of cellular glutathione, 
      inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, and 
      loss in cell viability with halfmaximal effects observed for MCA concentrations 
      between 0.3 and 3 mM. In contrast, the presence of acetate, DCA, or TCA in a 
      concentration of 10 mM did not compromise cell viability nor affect cellular 
      glutathione content or GAPDH activity. However, the presence of DCA and TCA 
      significantly lowered the rate of cellular lactate production in viable 
      astrocytes. These data demonstrate that the extent of chlorination strongly 
      determines the potential of chlorinated acetates to disturb glutathione and/or 
      glucose metabolism of astrocytes.
FAU - Schmidt, Maike M
AU  - Schmidt MM
AD  - Center for Biomolecular Interactions Bremen, University of Bremen, P.O. Box 
      330440, 28334 Bremen, Germany.
FAU - Rohwedder, Astrid
AU  - Rohwedder A
FAU - Dringen, Ralf
AU  - Dringen R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100714
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 0 (Acetates)
RN  - 5GD84Y125G (chloroacetic acid)
RN  - 5V2JDO056X (Trichloroacetic Acid)
RN  - 9LSH52S3LQ (Dichloroacetic Acid)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Acetates/*toxicity
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Dichloroacetic Acid/toxicity
MH  - Glutathione/*metabolism
MH  - Glycolysis/drug effects/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Trichloroacetic Acid/toxicity
EDAT- 2010/07/16 06:00
MHDA- 2012/01/21 06:00
CRDT- 2010/07/15 06:00
PHST- 2010/04/20 00:00 [received]
PHST- 2010/07/01 00:00 [accepted]
PHST- 2010/06/29 00:00 [revised]
PHST- 2010/07/15 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2012/01/21 06:00 [medline]
AID - 10.1007/s12640-010-9209-8 [doi]
PST - ppublish
SO  - Neurotox Res. 2011 May;19(4):628-37. doi: 10.1007/s12640-010-9209-8. Epub 2010 
      Jul 14.