PMID- 20629970
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20160511
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Linking)
VI  - 57
IP  - 5
DP  - 2010 Oct
TI  - Pathogenicity and molecular characterization of emerging porcine reproductive and 
      respiratory syndrome virus in Vietnam in 2007.
PG  - 315-29
LID - 10.1111/j.1865-1682.2010.01152.x [doi]
AB  - In 2007, Vietnam experienced swine disease outbreaks causing clinical signs 
      similar to the 'porcine high fever disease' that occurred in China during 2006. 
      Analysis of diagnostic samples from the disease outbreaks in Vietnam identified 
      porcine reproductive and respiratory syndrome virus (PRRSV) and porcine 
      circovirus type 2 (PCV-2). Additionally, Escherichia coli and Streptococcus equi 
      subspecies zooepidemicus were cultured from lung and spleen, and Streptococcus 
      suis from one spleen sample. Genetic characterization of the Vietnamese PRRSV 
      isolates revealed that this virus belongs to the North American genotype (type 2) 
      with a high nucleotide identity to the recently reported Chinese strains. Amino 
      acid sequence in the nsp2 region revealed 95.7-99.4% identity to Chinese strain 
      HUN4, 68-69% identity to strain VR-2332 and 58-59% identity to strain MN184. A 
      partial deletion in the nsp2 gene was detected; however, this deletion did not 
      appear to enhance the virus pathogenicity in the inoculated pigs. Animal 
      inoculation studies were conducted to determine the pathogenicity of PRRSV and to 
      identify other possible agents present in the original specimens. Pigs inoculated 
      with PRRSV alone and their contacts showed persistent fever, and two of five pigs 
      developed cough, neurological signs and swollen joints. Necropsy examination 
      showed mild to moderate bronchopneumonia, enlarged lymph nodes, fibrinous 
      pericarditis and polyarthritis. PRRSV was re-isolated from blood and tissues of 
      the inoculated and contact pigs. Pigs inoculated with lung and spleen tissue 
      homogenates from sick pigs from Vietnam developed high fever, septicaemia, and 
      died acutely within 72 h, while their contact pigs showed no clinical signs 
      throughout the experiment. Streptococcus equi subspecies zooepidemicus was 
      cultured, and PRRSV was re-isolated only from the inoculated pigs. Results 
      suggest that the cause of the swine deaths in Vietnam is a multifactorial 
      syndrome with PRRSV as a major factor.
CI  - (c) 2010 Blackwell Verlag GmbH.
FAU - Metwally, S
AU  - Metwally S
AD  - FAO Reference Center for Vesicular Diseases, USDA, APHIS, Foreign Animal Disease 
      Diagnostic Laboratory, National Veterinary Service Laboratories (NVSL), Plum 
      Island Animal Disease Center (PIADC), Greenport, NY, USA. 
      Samia.A.Metwally@aphis.usda.gov
FAU - Mohamed, F
AU  - Mohamed F
FAU - Faaberg, K
AU  - Faaberg K
FAU - Burrage, T
AU  - Burrage T
FAU - Prarat, M
AU  - Prarat M
FAU - Moran, K
AU  - Moran K
FAU - Bracht, A
AU  - Bracht A
FAU - Mayr, G
AU  - Mayr G
FAU - Berninger, M
AU  - Berninger M
FAU - Koster, L
AU  - Koster L
FAU - To, T L
AU  - To TL
FAU - Nguyen, V L
AU  - Nguyen VL
FAU - Reising, M
AU  - Reising M
FAU - Landgraf, J
AU  - Landgraf J
FAU - Cox, L
AU  - Cox L
FAU - Lubroth, J
AU  - Lubroth J
FAU - Carrillo, C
AU  - Carrillo C
LA  - eng
PT  - Journal Article
DEP - 20100709
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
SB  - IM
MH  - Animals
MH  - Arthritis/pathology
MH  - Communicable Diseases, Emerging/epidemiology/*veterinary
MH  - Disease Outbreaks/veterinary
MH  - Lymph Nodes/pathology
MH  - Pericardium/pathology
MH  - Phylogeny
MH  - Pneumonia/epidemiology
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*pathogenicity
MH  - Swine
MH  - Vietnam/epidemiology
EDAT- 2010/07/16 06:00
MHDA- 2011/02/23 06:00
CRDT- 2010/07/16 06:00
PHST- 2010/07/16 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
AID - JVA1152 [pii]
AID - 10.1111/j.1865-1682.2010.01152.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2010 Oct;57(5):315-29. doi: 
      10.1111/j.1865-1682.2010.01152.x. Epub 2010 Jul 9.