PMID- 20630983
OWN - NLM
STAT- MEDLINE
DCOM- 20101222
LR  - 20121115
IS  - 1477-0393 (Electronic)
IS  - 0748-2337 (Linking)
VI  - 26
IP  - 10
DP  - 2010 Nov
TI  - Free fatty acids profile of the fetal brain and the plasma, liver, brain and 
      kidneys of pregnant rats treated with sodium arsenite at mid-organogenesis.
PG  - 657-66
LID - 10.1177/0748233710375952 [doi]
AB  - Free fatty acids (FFAs) are known to be markers of cellular membrane degradation 
      through lipid peroxidation and are substrates for the production of reactive 
      oxygen species (ROS). Oxidative stress, due to overproduction of ROS, may 
      facilitate cellular insult by various toxicants. The ability of the rat conceptus 
      to respond to toxic stress may be critical for normal development. In this study, 
      the effects of the environmental toxicant sodium arsenite (NaAsO(2)) on FFAs were 
      investigated after administering a single oral dose, in water and in a lipid 
      medium, to pregnant rats on gestational day (GD) 10, a time point at 
      mid-organogenesis. NaAsO(2) was administered in deionized water (AsH(2)O) or in half 
      and half dairy cream (AsHH) at a dose of 41 mg sodium arsenite (NaAsO(2))/kg body 
      weight. Control animals were treated with either dairy cream (HH) or deionized 
      water (H(2)O). The animals were sacrificed on GD 20. The fetal brain and the 
      maternal liver, brain, plasma and kidneys were harvested. The FFAs were extracted 
      and analyzed by gas chromatography. In the liver, there was an increase of 
      myristic acid (1200%), myristoleic acid (174%), palmitic acid (47%), elaidic acid 
      (456%), oleic acid (165%) and docosahexaenoic acid (224%) in the AsH(2)O group as 
      compared to the AsHH group. Oleic acid and arachidonic acid were increased by 
      192% and 900%, respectively, in the AsH(2)O group as compared to the H(2)O group, and 
      myristic acid was decreased by 90% in the AsHH group as compared to the HH group. 
      In the maternal brain, myristoleic acid was decreased by 91% in the AsH(2)O group 
      as compared to the H(2)O group, and DHA increased by 148% in the AsHH group as 
      compared to the HH group. In the fetal brain, myristic and stearic acids were 
      decreased by 87% and 89%, respectively, in the AsH(2)O group as compared to the 
      AsHH group. Myristic, stearic and arachidonic acids were increased by 411%, 265%, 
      and 144%, respectively, in the AsHH group as compared to the HH group. There was 
      no effect on the fatty acids concentrations in the kidney or plasma as compared 
      to controls. This study shows that NaAsO(2) produced a differential effect on the 
      fatty acid profiles in rats. Further investigation is needed to elucidate the 
      role of fatty acids in differential signaling and regulation by either the 
      palmitoylation or myristoylation process of cellular functions in these target 
      organs.
FAU - Ross, Ivan A
AU  - Ross IA
AD  - Center for Food Safety and Applied Nutrition, Division of Toxicology, Office of 
      Applied Research and Safety Assessment, Laurel, MD, USA. rossivan@msn.com
FAU - Boyle, Thomas
AU  - Boyle T
FAU - Johnson, Widmark D
AU  - Johnson WD
FAU - Sprando, Robert L
AU  - Sprando RL
FAU - O'Donnell, Michael W
AU  - O'Donnell MW
FAU - Ruggles, Dennis
AU  - Ruggles D
FAU - Kim, Chung S
AU  - Kim CS
LA  - eng
PT  - Journal Article
DEP - 20100714
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (Arsenites)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fatty Acids)
RN  - 0 (Sodium Compounds)
RN  - 48OVY2OC72 (sodium arsenite)
SB  - IM
MH  - Animals
MH  - Arsenites/*toxicity
MH  - Brain/embryology/metabolism
MH  - Environmental Pollutants/*toxicity
MH  - Fatty Acids/blood/*metabolism
MH  - Female
MH  - Fetus/embryology/*metabolism
MH  - Kidney/embryology/metabolism
MH  - Liver/embryology/metabolism
MH  - Organogenesis/*drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sodium Compounds/*toxicity
EDAT- 2010/07/16 06:00
MHDA- 2010/12/24 06:00
CRDT- 2010/07/16 06:00
PHST- 2010/07/16 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2010/12/24 06:00 [medline]
AID - 0748233710375952 [pii]
AID - 10.1177/0748233710375952 [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2010 Nov;26(10):657-66. doi: 10.1177/0748233710375952. Epub 
      2010 Jul 14.