PMID- 20631119
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 19
DP  - 2010 Oct
TI  - Two overlapping domains of a lyssavirus matrix protein that acts on different 
      cell death pathways.
PG  - 9897-906
LID - 10.1128/JVI.00761-10 [doi]
AB  - The lyssavirus matrix (M) protein induces apoptosis. The regions of the M protein 
      that are essential for triggering cell death pathways are not yet clearly 
      defined. We therefore compared the M proteins from two viruses that have 
      contrasting characteristics in terms of cellular apoptosis: a genotype 3 
      lyssavirus, Mokola virus (MOK), and a genotype 1 rabies virus isolated from a dog 
      from Thailand (THA). We identified a 20-amino-acid fragment (corresponding to 
      positions 67 to 86) that retained the cell death activities of the full-length M 
      protein from MOK via both the tumor necrosis factor-related apoptosis-inducing 
      ligand (TRAIL) and inhibition of cytochrome c oxidase (CcO) activity. We found 
      that the amino acids at positions 77 and 81 have an essential role in triggering 
      these two cell death pathways. Directed mutagenesis demonstrated that the amino 
      acid at position 77 affects CcO activity, whereas the amino acid at position 81 
      affects TRAIL-dependent apoptosis. Mutations in the full-length M protein that 
      compromised induction of either of these two pathways resulted in delayed 
      apoptosis compared with the time to apoptosis for the nonmutated control.
FAU - Larrous, Florence
AU  - Larrous F
AD  - Institut Pasteur, Unite Dynamique des lyssavirus et adaptation a l'hote, 75724 
      Paris Cedex 15, France.
FAU - Gholami, Alireza
AU  - Gholami A
FAU - Mouhamad, Shahul
AU  - Mouhamad S
FAU - Estaquier, Jerome
AU  - Estaquier J
FAU - Bourhy, Herve
AU  - Bourhy H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100714
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFSF10 protein, human)
RN  - 0 (Viral Matrix Proteins)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Apoptosis/physiology
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics
MH  - Dogs
MH  - Electron Transport Complex IV/antagonists & inhibitors
MH  - HeLa Cells
MH  - Host-Pathogen Interactions/physiology
MH  - Humans
MH  - Lyssavirus/genetics/pathogenicity/*physiology
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Protein Structure, Tertiary
MH  - Rabies virus/genetics/pathogenicity/physiology
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - TNF-Related Apoptosis-Inducing Ligand/physiology
MH  - Viral Matrix Proteins/*chemistry/genetics/*physiology
PMC - PMC2937802
EDAT- 2010/07/16 06:00
MHDA- 2010/09/30 06:00
PMCR- 2011/04/01
CRDT- 2010/07/16 06:00
PHST- 2010/07/16 06:00 [entrez]
PHST- 2010/07/16 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
PHST- 2011/04/01 00:00 [pmc-release]
AID - JVI.00761-10 [pii]
AID - 0761-10 [pii]
AID - 10.1128/JVI.00761-10 [doi]
PST - ppublish
SO  - J Virol. 2010 Oct;84(19):9897-906. doi: 10.1128/JVI.00761-10. Epub 2010 Jul 14.