PMID- 20635439
OWN - NLM
STAT- MEDLINE
DCOM- 20101101
LR  - 20220409
IS  - 1976-2437 (Electronic)
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 51
IP  - 5
DP  - 2010 Sep
TI  - Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) 
      and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat 
      hippocampus.
PG  - 661-71
LID - 10.3349/ymj.2010.51.5.661 [doi]
AB  - PURPOSE: The purpose of this study is to explore the dynamic change of 
      brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine 
      kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress 
      conditions and to explore the influence of senescence on the productions 
      expression. MATERIALS AND METHODS: By using forced-swimming in 4 degrees C cold 
      ice water and 25 degrees C warm water, young and aged male rats were randomly 
      divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, 
      respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by 
      using Western-blotting and reverse transcription-polymerase chain reaction 
      (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress 
      session. RESULTS: The short AS induced a significant increase in BDNF mRNA and 
      protein in both age groups, but the changes in the young group were substantially 
      greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease 
      in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young 
      and age groups. The expression of BDNF mRNA and protein in the AS groups were 
      higher than in the CMRS groups at 15, 30, and 60 min after stress. CONCLUSION: 
      The results indicated that the up/down-regulation of BDNF and TrkB were affected 
      by aging and the stimulus paradigm, which might reflect important mechanisms by 
      which the hippocampus copes with stressful stimuli.
FAU - Shi, Shou-Sen
AU  - Shi SS
AD  - Department of Medical Psychology, Institute of Biochemistry and Molecular 
      Biology, Binzhou Medical University, Yantai, Shandong, China.
FAU - Shao, Shu-Hong
AU  - Shao SH
FAU - Yuan, Bang-Ping
AU  - Yuan BP
FAU - Pan, Fang
AU  - Pan F
FAU - Li, Zun-Ling
AU  - Li ZL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Corticosterone/blood
MH  - *Gene Expression Regulation
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Radioimmunoassay
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stress, Physiological/genetics/*physiology
PMC - PMC2908888
COIS- The authors have no financial conflicts of interest.
EDAT- 2010/07/17 06:00
MHDA- 2010/11/03 06:00
PMCR- 2010/09/01
CRDT- 2010/07/17 06:00
PHST- 2010/07/17 06:00 [entrez]
PHST- 2010/07/17 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - 201009661 [pii]
AID - 10.3349/ymj.2010.51.5.661 [doi]
PST - ppublish
SO  - Yonsei Med J. 2010 Sep;51(5):661-71. doi: 10.3349/ymj.2010.51.5.661.