PMID- 20637549
OWN - NLM
STAT- MEDLINE
DCOM- 20110506
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 147
IP  - 1-2
DP  - 2011 Jan 10
TI  - Effect of an interferon-stimulated response element (ISRE) mutant of porcine 
      circovirus type 2 (PCV2) on PCV2-induced pathological lesions in a porcine 
      reproductive and respiratory syndrome virus (PRRSV) co-infection model.
PG  - 49-58
LID - 10.1016/j.vetmic.2010.06.010 [doi]
AB  - Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine 
      circovirus-associated diseases (PCVAD) in swine. Coinfections of PCV2 with other 
      swine pathogens increase the severity of PCVAD. Induction of proinflammatory 
      cytokines by coinfecting pathogens may attribute to the exacerbation of PCVAD 
      during coinfections. An interferon-stimulated response element (ISRE) sequence 
      was identified in the origin of replication of PCV2 genome. To assess the role of 
      ISRE in PCV2 pathogenesis during coinfection, an ISRE-mutant PCV2 was constructed 
      and used to experimentally infect pigs with either ISRE mutant or wildtype PCV2 
      singly or in combination with porcine reproductive and respiratory syndrome virus 
      (PRRSV). The results showed that, during early stage of infection at 14 days 
      post-inoculation (dpi), the ISRE mutation reduced viral replication and elicited 
      low antibody responses. However, at 28 dpi viremia in pigs infected with the 
      ISRE-mutant was on an upward trend, and microscopic lesion scores in pigs 
      inoculated with the ISRE-mutant were significantly more severe than in wildtype 
      PCV2-infected pigs. Coinfection with PRRSV caused an opposite shift in the in 
      vivo dynamics of the ISRE-mutant at 14 dpi with the lymph node histopathological 
      lesions being significantly more severe in pigs coinfected with the ISRE-mutant 
      PCV2 and PRRSV than in pigs coinfected with wildtype PCV2 and PRRSV. PCV2 genomic 
      copy numbers in pigs coinfected with ISRE-mutant and PRRSV were also higher than 
      those coinfected with wildtype PCV2 and PRRSV. The results suggested that the 
      ISRE element in PCV2 genome may play a potential role in viral pathogenesis.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Ramamoorthy, S
AU  - Ramamoorthy S
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 
      24061-0913, USA.
FAU - Opriessnig, T
AU  - Opriessnig T
FAU - Pal, N
AU  - Pal N
FAU - Huang, F F
AU  - Huang FF
FAU - Meng, X J
AU  - Meng XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100619
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/analysis
MH  - Cell Line
MH  - Circoviridae Infections/complications/immunology/pathology/*veterinary/virology
MH  - Circovirus/genetics/immunology/*physiology
MH  - Genes, Viral/genetics
MH  - Haplorhini
MH  - Interferons/metabolism
MH  - Lung/pathology
MH  - Lymph Nodes/pathology
MH  - Microbial Viability/genetics
MH  - Mutation
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*physiology
MH  - Random Allocation
MH  - Swine
MH  - Swine Diseases/*pathology/*virology
MH  - Time Factors
MH  - Viral Load
EDAT- 2010/07/20 06:00
MHDA- 2011/05/07 06:00
CRDT- 2010/07/20 06:00
PHST- 2009/11/05 00:00 [received]
PHST- 2010/05/27 00:00 [revised]
PHST- 2010/06/14 00:00 [accepted]
PHST- 2010/07/20 06:00 [entrez]
PHST- 2010/07/20 06:00 [pubmed]
PHST- 2011/05/07 06:00 [medline]
AID - S0378-1135(10)00312-3 [pii]
AID - 10.1016/j.vetmic.2010.06.010 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 Jan 10;147(1-2):49-58. doi: 10.1016/j.vetmic.2010.06.010. 
      Epub 2010 Jun 19.