PMID- 20638501
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20141120
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 674
IP  - 1
DP  - 2010 Jul 26
TI  - Simultaneous determination of phoxim and its photo-transformation metabolite 
      residues in eggs using liquid chromatography coupled with electrospray ionization 
      tandem mass spectrometry.
PG  - 64-70
LID - 10.1016/j.aca.2010.06.016 [doi]
AB  - The principal objective of this study was to develop an appropriate, sensitive, 
      and selective method for the simultaneous quantitative determination of phoxim 
      and its photo-transformation product, O,O-diethyl 
      alpha-cyanobenzylideneamino-thiophosphonate (DCTP) in both chicken and quail 
      eggs. Eggs (1 g) were blended with anhydrous magnesium sulfate (1 g) for sample 
      pretreatment and extracted with acetonitrile. The extracts were then further 
      purified with SPE silica gel tubes deactivated with trimethylamine. Residues were 
      analyzed via a reversed phase-liquid chromatography-tandem mass spectrometry 
      (RP-LC-MS/MS) in positive-ion electrospray ionization (ESI) mode. Tebufenozide 
      was utilized as an internal standard for the quantification of phoxim and its 
      metabolite residues. The identification and quantification of analytes were based 
      on ion transitions monitored by multiple reaction monitoring (MRM). LC-MS/MS 
      analysis was performed from 0.02 to 1 mg kg(-1) and correlation coefficients 
      (r(2)) ranging from 0.998 to 0.999 were obtained for both analytes in blank egg 
      extracts. The relative standard deviations (RSDs) of intra- and inter-day 
      variations ranged from 2.1% to 6.7% and from 2.8% to 6.4% for phoxim and DCTP in 
      chicken and quail eggs. At all levels of fortification (0.02, 0.05, and 0.125 mg 
      kg(-1)), the recoveries fell within a range of 81.3% to 93.6% for phoxim and 
      83.3% to 90.1% for DCTP. The matrix effect was <2%, due to the partial dilution 
      of the sample. Decision limits (CCalpha) and detection capabilities (CCbeta) were 
      in the range of 0.0005-0.0044 and 0.0054-0.0224 mg kg(-1), respectively. The 
      method was evaluated further by analyzing real samples purchased from markets. 
      All chicken and quail egg samples were free from residues of the target 
      compounds.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Lee, Jung Han
AU  - Lee JH
AD  - Department of Chemistry and Research Institute of Life Science, Gyeongsang 
      National University, Jinju 660-701, Republic of Korea.
FAU - Park, Semin
AU  - Park S
FAU - Jeong, Won Young
AU  - Jeong WY
FAU - Park, Hyung Jin
AU  - Park HJ
FAU - Kim, Hae Gyeong
AU  - Kim HG
FAU - Lee, Soo-Jung
AU  - Lee SJ
FAU - Shim, Jae-Han
AU  - Shim JH
FAU - Kim, Soo Taek
AU  - Kim ST
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
FAU - Im, Moo Hyeog
AU  - Im MH
FAU - Choi, Ok Ja
AU  - Choi OJ
FAU - Shin, Sung Chul
AU  - Shin SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100623
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Organothiophosphorus Compounds)
RN  - 0 (Pesticide Residues)
RN  - 6F5V775VPO (phoxim)
SB  - IM
MH  - Chromatography, Reverse-Phase
MH  - Eggs/*analysis
MH  - Organothiophosphorus Compounds/*analysis/metabolism
MH  - Pesticide Residues/*analysis/metabolism
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2010/07/20 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/07/20 06:00
PHST- 2010/04/15 00:00 [received]
PHST- 2010/06/13 00:00 [revised]
PHST- 2010/06/14 00:00 [accepted]
PHST- 2010/07/20 06:00 [entrez]
PHST- 2010/07/20 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0003-2670(10)00754-3 [pii]
AID - 10.1016/j.aca.2010.06.016 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2010 Jul 26;674(1):64-70. doi: 10.1016/j.aca.2010.06.016. Epub 
      2010 Jun 23.