PMID- 20638776
OWN - NLM
STAT- MEDLINE
DCOM- 20110120
LR  - 20100920
IS  - 1432-0436 (Electronic)
IS  - 0301-4681 (Linking)
VI  - 80
IP  - 2-3
DP  - 2010 Sep-Oct
TI  - Effect of transforming growth factor alpha overexpression on urogenital organ 
      development in mouse.
PG  - 82-8
LID - 10.1016/j.diff.2010.06.006 [doi]
AB  - Transforming growth factor-alpha (TGFalpha) promotes cell proliferation by binding to the 
      epidermal growth factor receptor (EGFR). TGFalpha and EGFR overexpression have been 
      reported in various human cancers. However, whether TGFalpha induces cancer by itself 
      is unknown in urogenital organs. To investigate whether TGFalpha overexpression 
      induces carcinogenesis in urogenital organs, we analyzed the phenotypes of 
      urogenital organs in male TGFalpha transgenic (TG) mice of the CD1 strain. Urogenital 
      organs including the kidney, bladder, prostate, seminal vesicles, testes, and 
      epididymis were isolated from 4- to 48-week-old TGFalpha TG and wild-type (WT) CD1 
      mice. Prostates were separated into anterior prostate (AP), dorsolateral prostate 
      (DLP), and ventral prostate (VP). Neither tumor formation nor epithelial 
      hyperplasia was observed in the TGFalpha TG mouse urogenital organs that we have 
      investigated. Histopathologically, in prostate, we found an increased number of 
      p63-positive basal epithelial cells in the TGFalpha TG mice AP and DLP. There was no 
      morphological change in the stromal component, such as hypercellular stroma or 
      fibrosis. However, bladder weight was greater in TGFalpha TG mice than that in WT 
      mice, and distended bladders were observed macroscopically in 19 of 20 TGFalpha TG 
      mice over 20 weeks of age. Ki67 labeling index was increased significantly in the 
      TGFalpha TG mouse urethral epithelium, whereas neither epithelial hyperplasia nor 
      hypertrophy was observed. In conclusion, our results suggest that TGFalpha 
      overexpression in mouse urogenital organs alone may not be responsible for tumor 
      formation and epithelial hyperplasia, but is involved in bladder outlet 
      obstruction.
CI  - Copyright (c) 2010 International Society of Differentiation. Published by Elsevier 
      B.V. All rights reserved.
FAU - Yoshio, Yuko
AU  - Yoshio Y
AD  - Department of Nephro-Urologic Surgery and Andrology, Mie University Graduate 
      School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
FAU - Ishii, Kenichiro
AU  - Ishii K
FAU - Arase, Shigeki
AU  - Arase S
FAU - Hori, Yasuhide
AU  - Hori Y
FAU - Nishikawa, Kohei
AU  - Nishikawa K
FAU - Soga, Norihito
AU  - Soga N
FAU - Kise, Hideaki
AU  - Kise H
FAU - Arima, Kiminobu
AU  - Arima K
FAU - Sugimura, Yoshiki
AU  - Sugimura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100717
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (DNA Primers)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Polymerase Chain Reaction
MH  - Transforming Growth Factor alpha/*genetics
MH  - Urogenital System/*embryology
EDAT- 2010/07/20 06:00
MHDA- 2011/01/21 06:00
CRDT- 2010/07/20 06:00
PHST- 2010/01/27 00:00 [received]
PHST- 2010/06/25 00:00 [revised]
PHST- 2010/06/29 00:00 [accepted]
PHST- 2010/07/20 06:00 [entrez]
PHST- 2010/07/20 06:00 [pubmed]
PHST- 2011/01/21 06:00 [medline]
AID - S0301-4681(10)00069-1 [pii]
AID - 10.1016/j.diff.2010.06.006 [doi]
PST - ppublish
SO  - Differentiation. 2010 Sep-Oct;80(2-3):82-8. doi: 10.1016/j.diff.2010.06.006. Epub 
      2010 Jul 17.