PMID- 20639774
OWN - NLM
STAT- MEDLINE
DCOM- 20110303
LR  - 20100902
IS  - 1536-4801 (Electronic)
IS  - 0277-2116 (Linking)
VI  - 51
IP  - 3
DP  - 2010 Sep
TI  - Increased HLA-DR expression on tissue eosinophils in eosinophilic esophagitis.
PG  - 290-4
LID - 10.1097/MPG.0b013e3181e083e7 [doi]
AB  - OBJECTIVE: The aim of the study was to investigate whether eosinophils have 
      increased human leukocyte antigen (HLA)-DR expression in subjects with 
      eosinophilic esophagitis (EoE) compared with controls. PATIENTS AND METHODS: 
      Patients who were undergoing an upper endoscopy with biopsies for suspected 
      gastroesophageal reflux disease (GERD) or EoE at Lucile Packard Children's 
      Hospital were enrolled. In total, the blood and tissue samples of 10 healthy 
      controls (HC), 11 subjects with GERD, and 10 with EoE were studied. Multiple 
      tissue staining to identify eosinophils (via eosinophil cationic protein-clone 
      EG2) and major histocompatibility complex class II cell surface receptors (via 
      HLA-DR) was performed via immunohistochemistry. The peripheral blood was analyzed 
      using flow cytometry to detect eosinophil HLA-DR expression among these subjects. 
      RESULTS: In the tissue, a greater proportion of eosinophils expressed HLA-DR 
      among the subjects with EoE (mean 0.83 +/- 0.14, n = 9) relative to those with 
      GERD (mean 0.18 +/- 0.19, n = 8, P < 0.01) and HC (mean 0.18 +/- 0.13, n = 6, P < 
      0.01). In total, 6 participants (4 HC subjects and 2 subjects with GERD) did not 
      have any eosinophils identified on tissue staining and were unable to be included 
      in the present statistical analysis. In the blood, there was no statistically 
      significant difference in eosinophil HLA-DR expression among HC subjects (mean 
      415 +/- 217, n = 6), subjects with GERD (mean 507 +/- 429, n = 2), and those with 
      EoE (mean 334 +/- 181, n = 6). CONCLUSIONS: These data demonstrate that the 
      eosinophils from the esophagus of subjects with EoE have increased HLA-DR 
      expression within this tissue.
FAU - Patel, Anup J
AU  - Patel AJ
AD  - Lucile Packard Children's Hospital, Stanford University, Palo Alto, CA, USA. 
      drajpatel@gmail.com
FAU - Fuentebella, Judy
AU  - Fuentebella J
FAU - Gernez, Yael
AU  - Gernez Y
FAU - Nguyen, Tammie
AU  - Nguyen T
FAU - Bass, Dorsey
AU  - Bass D
FAU - Berquist, William
AU  - Berquist W
FAU - Cox, Kenneth
AU  - Cox K
FAU - Sibley, Eric
AU  - Sibley E
FAU - Kerner, John
AU  - Kerner J
FAU - Nadeau, Kari
AU  - Nadeau K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pediatr Gastroenterol Nutr
JT  - Journal of pediatric gastroenterology and nutrition
JID - 8211545
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Eosinophilic Esophagitis/*immunology/metabolism
MH  - Eosinophils/immunology/*metabolism
MH  - Esophagus/*immunology/metabolism
MH  - Female
MH  - Gastroesophageal Reflux/*immunology/metabolism
MH  - HLA-DR Antigens/*metabolism
MH  - Humans
MH  - Infant
MH  - Male
MH  - Young Adult
EDAT- 2010/07/20 06:00
MHDA- 2011/03/04 06:00
CRDT- 2010/07/20 06:00
PHST- 2010/07/20 06:00 [entrez]
PHST- 2010/07/20 06:00 [pubmed]
PHST- 2011/03/04 06:00 [medline]
AID - 10.1097/MPG.0b013e3181e083e7 [doi]
PST - ppublish
SO  - J Pediatr Gastroenterol Nutr. 2010 Sep;51(3):290-4. doi: 
      10.1097/MPG.0b013e3181e083e7.