PMID- 20641033
OWN - NLM
STAT- MEDLINE
DCOM- 20110330
LR  - 20211020
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 26
IP  - 1
DP  - 2011 Jan
TI  - Polymorphisms in predicted miRNA binding sites and osteoporosis.
PG  - 72-8
LID - 10.1002/jbmr.186 [doi]
AB  - MicroRNAs (miRNAs) regulate posttranscriptional gene expression usually by 
      binding to 3'-untranslated regions (3'-UTRs) of target message RNAs (mRNAs). 
      Hence genetic polymorphisms on 3'-UTRs of mRNAs may alter binding affinity 
      between miRNAs target 3'-UTRs, thereby altering translational regulation of 
      target mRNAs and/or degradation of mRNAs, leading to differential protein 
      expression of target genes. Based on a database that catalogues predicted 
      polymorphisms in miRNA target sites (poly-miRTSs), we selected 568 polymorphisms 
      within 3'-UTRs of target mRNAs and performed association analyses between these 
      selected poly-miRTSs and osteoporosis in 997 white subjects who were genotyped by 
      Affymetrix Human Mapping 500K arrays. Initial discovery (in the 997 subjects) and 
      replication (in 1728 white subjects) association analyses identified three 
      poly-miRTSs (rs6854081, rs1048201, and rs7683093) in the fibroblast growth factor 
      2 (FGF2) gene that were significantly associated with femoral neck bone mineral 
      density (BMD). These three poly-miRTSs serve as potential binding sites for 9 
      miRNAs (eg, miR-146a and miR-146b). Further gene expression analyses demonstrated 
      that the FGF2 gene was differentially expressed between subjects with high versus 
      low BMD in three independent sample sets. Our initial and replicate association 
      studies and subsequent gene expression analyses support the conclusion that these 
      three polymorphisms of the FGF2 gene may contribute to susceptibility to 
      osteoporosis, most likely through their effects on altered binding affinity for 
      specific miRNAs.
CI  - (c) 2011 American Society for Bone and Mineral Research.
FAU - Lei, Shu-Feng
AU  - Lei SF
AD  - Laboratory of Molecular and Statistical, College of Life Sciences, Hunan Normal 
      University, Changsha, Hunan, People's Republic of China.
FAU - Papasian, Christopher J
AU  - Papasian CJ
FAU - Deng, Hong-Wen
AU  - Deng HW
LA  - eng
GR  - R01AR050496/AR/NIAMS NIH HHS/United States
GR  - RC2 DE020756/DE/NIDCR NIH HHS/United States
GR  - R01 AG026564/AG/NIA NIH HHS/United States
GR  - P50AR055081/AR/NIAMS NIH HHS/United States
GR  - RC2DE020756/DE/NIDCR NIH HHS/United States
GR  - R01 AR057049/AR/NIAMS NIH HHS/United States
GR  - P50 AR055081/AR/NIAMS NIH HHS/United States
GR  - R03 TW008221/TW/FIC NIH HHS/United States
GR  - R01 AR050496/AR/NIAMS NIH HHS/United States
GR  - R01AG026564/AG/NIA NIH HHS/United States
GR  - R03TW008221/TW/FIC NIH HHS/United States
GR  - R01AR057049/AR/NIAMS NIH HHS/United States
GR  - CAPMC/CIHR/Canada
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (MicroRNAs)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Binding Sites
MH  - Bone Density/genetics
MH  - Female
MH  - Femur Neck/physiopathology
MH  - Fibroblast Growth Factor 2/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Osteoporosis/*genetics/physiopathology
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Reproducibility of Results
PMC - PMC3179316
EDAT- 2010/07/20 06:00
MHDA- 2011/03/31 06:00
PMCR- 2012/01/01
CRDT- 2010/07/20 06:00
PHST- 2010/07/20 06:00 [entrez]
PHST- 2010/07/20 06:00 [pubmed]
PHST- 2011/03/31 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 10.1002/jbmr.186 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2011 Jan;26(1):72-8. doi: 10.1002/jbmr.186.