PMID- 20643487
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20131121
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 227
IP  - 1-2
DP  - 2010 Oct 8
TI  - Central NOS inhibition differentially affects vasopressin gene expression in 
      hypothalamic nuclei in septic rats.
PG  - 80-6
LID - 10.1016/j.jneuroim.2010.06.019 [doi]
AB  - Our aim was to investigate the effect of central NOS inhibition on hypothalamic 
      arginine vasopressin (AVP) gene expression, hormone release and on the 
      cardiovascular response during experimental sepsis. Male Wistar rats were 
      intracerebroventricularly injected with the non-selective NO synthase (NOS) 
      inhibitor (L-NAME) or aminoguanidine, a selective inhibitor of the inducible 
      isoform (iNOS). After 30 min, sepsis was induced by cecal ligation and puncture 
      (CLP) causing an increase in heart rate (HR), as well as a reduction in median 
      arterial pressure (MAP) and AVP expression ratio (AVP(R)), mainly in the 
      supraoptic nucleus. AVP plasma levels (AVPp) increased in the early but not in 
      the late phase of sepsis. L-NAME pretreatment increased MAP but did not change 
      HR. It also resulted in an increase in AVPp at all time points, except 24h, when 
      it returned to basal levels. AVP(R), however remained reduced in both nuclei. 
      Aminoguanidine pretreatment resulted in increased MAP in the early phase and 
      higher AVP(R) in the supraoptic, but not in the paraventricular nucleus, while 
      AVPp remained elevated at all time points. We suggest that increased central NO 
      production, mainly inducible NOS-derived, reduces AVP gene expression 
      differentially in supraoptic and paraventricular nuclei, and that this may 
      contribute to low AVP plasma levels and hypotension in the late phase of sepsis.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Oliveira-Pelegrin, Gabriela Ravanelli
AU  - Oliveira-Pelegrin GR
AD  - Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia 
      de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.
FAU - de Azevedo, Sergio Vicente
AU  - de Azevedo SV
FAU - Yao, Song Tieng
AU  - Yao ST
FAU - Murphy, David
AU  - Murphy D
FAU - Rocha, Maria Jose Alves
AU  - Rocha MJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100718
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/*biosynthesis/*genetics
MH  - *Gene Expression Regulation/immunology
MH  - Injections, Intraventricular
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/administration & dosage
MH  - Nitric Oxide/antagonists & inhibitors/metabolism
MH  - Nitric Oxide Synthase Type II/*antagonists & inhibitors/*physiology
MH  - Paraventricular Hypothalamic Nucleus/*enzymology/immunology/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Sepsis/enzymology/*immunology/pathology
MH  - Supraoptic Nucleus/*enzymology/immunology/pathology
EDAT- 2010/07/21 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/07/21 06:00
PHST- 2010/03/08 00:00 [received]
PHST- 2010/06/16 00:00 [revised]
PHST- 2010/06/21 00:00 [accepted]
PHST- 2010/07/21 06:00 [entrez]
PHST- 2010/07/21 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - S0165-5728(10)00286-9 [pii]
AID - 10.1016/j.jneuroim.2010.06.019 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2010 Oct 8;227(1-2):80-6. doi: 10.1016/j.jneuroim.2010.06.019. 
      Epub 2010 Jul 18.