PMID- 20644585
OWN - NLM
STAT- MEDLINE
DCOM- 20100805
LR  - 20210201
IS  - 0132-3423 (Print)
IS  - 0132-3423 (Linking)
VI  - 36
IP  - 3
DP  - 2010 May-Jun
TI  - [Haponin (eIF1AD) interacts with glyceraldehyde 3-phosphate dehydrogenase in the 
      CHO-K1 cell line].
PG  - 312-8
AB  - Haponin (HLDF-alike protein) was previously identified from the human 
      promyelocytic leukemia HL-60 cell line. For the functional study of this protein, 
      we obtained recombinant haponin with an N-terminal hexahistidine tag using a 
      baculovirus expression system. Antibodies against 6xHis-haponin were produced, 
      and the expression of endogenous haponin was demonstrated in mammalian cell lines 
      of different origin. Using affinity chromatography and immunoprecipitation 
      methods, we have shown that in CHO-K1 cells haponin interacts with glyceraldehyde 
      3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes 
      with a diverse set of noncanonical functions.
FAU - Rakitina, T V
AU  - Rakitina TV
FAU - Bogatova, O V
AU  - Bogatova OV
FAU - Smirnova, E V
AU  - Smirnova EV
FAU - Pozdeev, V I
AU  - Pozdeev VI
FAU - Kostanian, I A
AU  - Kostanian IA
FAU - Lipkin, V M
AU  - Lipkin VM
LA  - rus
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Russia (Federation)
TA  - Bioorg Khim
JT  - Bioorganicheskaia khimiia
JID - 7804941
RN  - 0 (EIF1AD protein, human)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Line, Tumor
MH  - Cricetinae
MH  - Cricetulus
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Humans
MH  - Protein Interaction Mapping
MH  - Proteins/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/genetics/isolation & purification/metabolism
EDAT- 2010/07/21 06:00
MHDA- 2010/08/06 06:00
CRDT- 2010/07/21 06:00
PHST- 2010/07/21 06:00 [entrez]
PHST- 2010/07/21 06:00 [pubmed]
PHST- 2010/08/06 06:00 [medline]
AID - 36n3F312L318 [pii]
AID - 10.1134/s1068162010030027 [doi]
PST - ppublish
SO  - Bioorg Khim. 2010 May-Jun;36(3):312-8. doi: 10.1134/s1068162010030027.