PMID- 20645716
OWN - NLM
STAT- MEDLINE
DCOM- 20101130
LR  - 20100721
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 48
IP  - 4
DP  - 2010 Apr
TI  - Antifungal activity of extracts of some plants used in Brazilian traditional 
      medicine against the pathogenic fungus Paracoccidioides brasiliensis.
PG  - 388-96
LID - 10.3109/13880200903150385 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by 
      Paracoccidioides brasiliensis Almeida (Onygenales) that requires 1-2 years of 
      treatment. In the absence of drug therapy, the disease is usually fatal, 
      highlighting the need for the identification of safer, novel, and more effective 
      antifungal compounds. With this need in mind, several plants employed in 
      Brazilian traditional medicine were assayed on P. brasiliensis and murine 
      macrophages. Extracts were prepared from 10 plant species: Inga spp. Mill. 
      (Leguminosae), Schinus terebinthifolius Raddi (Anacardiaceae), Punica granatum L. 
      (Punicaceae), Alternanthera brasiliana Kuntze (Amaranthaceae), Piper regnellii 
      CDC. (Piperaceae), P. abutiloides Kunth (Piperaceae), Herissantia crispa L. Briz. 
      (Malvaceae), Rubus urticaefolius Poir (Rosaceae), Rumex acetosa L. 
      (Polygonaceae), and Baccharis dracunculifolia DC. (Asteraceae). Hexane fractions 
      from hydroalcoholic extracts of Piper regnellii and Baccharis dracunculifolia 
      were the most active against the fungus, displaying minimum inhibitory 
      concentration (MIC) values of 7.8 microg/mL and 7.8-30 mug/mL, respectively. 
      Additionally, neither of the extracts exhibited any apparent cytotoxic effects on 
      murine macrophages at 20 microg/mL. Analyses of these fractions using gas 
      chromatography-mass spectrometry (GC-MS) showed that the major components of B. 
      dracunculifolia were ethyl hydrocinnamate (14.35%) and spathulenol (16.02%), 
      while the major components of the hexane fraction of Piper regnellii were 
      1-methoxy-4-(1-propenyl) benzene (21.94%) and apiol (21.29%). The activities of 
      these fractions against P. brasiliensis without evidence of cytotoxicity to 
      macrophages justify their investigation as a potential source of new chemical 
      agents for the treatment of PCM.
FAU - Johann, Susana
AU  - Johann S
AD  - Laboratorio de Quimica dos Produtos Naturais, Centro de Pesquisas Rene Rachou, 
      Fiocruz, Belo Horizonte, MG, Brazil. sjohann@cpqrr.fiocruz.br
FAU - Cisalpino, Patricia Silva
AU  - Cisalpino PS
FAU - Watanabe, Gisele Almeida
AU  - Watanabe GA
FAU - Cota, Betania Barros
AU  - Cota BB
FAU - de Siqueira, Ezequias Pessoa
AU  - de Siqueira EP
FAU - Pizzolatti, Moacir Geraldo
AU  - Pizzolatti MG
FAU - Zani, Carlos Leomar
AU  - Zani CL
FAU - de Resende, Maria Aparecida
AU  - de Resende MA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Antifungal Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - *Antifungal Agents/adverse effects/isolation & purification/pharmacology
MH  - Brazil
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Macrophages/drug effects
MH  - Medicine, Traditional/*methods
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - Paracoccidioides/*drug effects/growth & development
MH  - *Plant Extracts/adverse effects/isolation & purification/pharmacology
MH  - Plants, Medicinal/*chemistry
EDAT- 2010/07/22 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/07/22 06:00
PHST- 2010/07/22 06:00 [entrez]
PHST- 2010/07/22 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.3109/13880200903150385 [doi]
PST - ppublish
SO  - Pharm Biol. 2010 Apr;48(4):388-96. doi: 10.3109/13880200903150385.