PMID- 20648658
OWN - NLM
STAT- MEDLINE
DCOM- 20110311
LR  - 20161125
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 88
IP  - 12
DP  - 2010 Sep
TI  - Appearance of phagocytic microglia adjacent to motoneurons in spinal cord tissue 
      from a presymptomatic transgenic rat model of amyotrophic lateral sclerosis.
PG  - 2736-46
LID - 10.1002/jnr.22424 [doi]
AB  - Microglial activation occurs early during the pathogenesis of amyotrophic lateral 
      sclerosis (ALS). Recent evidence indicates that the expression of mutant 
      Cu(2+)/Zn(2+) superoxide dismutase 1 (SOD1) in microglia contributes to the late 
      disease progression of ALS. However, the mechanism by which microglia influence 
      the neurodegenerative process and disease progression in ALS remains unclear. In 
      this study, we revealed that activated microglia aggregated in the lumbar spinal 
      cord of presymptomatic mutant SOD1(H46R) transgenic rats, an animal model of 
      familial ALS. The aggregated microglia expressed a marker of proliferating cell, 
      Ki67, and phagocytic marker proteins ED1 and major histocompatibility complex 
      (MHC) class II. The motoneurons near the microglial aggregates showed weak 
      choline acetyltransferase (ChAT) immunoreactivity and contained reduced granular 
      endoplasmic reticulum and altered nucleus electron microscopically. Furthermore, 
      immunopositive signals for tumor necrosis factor-alpha (TNFalpha) and monocyte 
      chemoattractant protein-1 (MCP-1) were localized in the aggregated microglia. 
      These results suggest that the activated and aggregated microglia represent 
      phagocytic features in response to early changes in motoneurons and possibly play 
      an important role in ALS disease progression during the presymptomatic stage.
FAU - Sanagi, Tomomi
AU  - Sanagi T
AD  - Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo 
      187-8502, Japan.
FAU - Yuasa, Shigeki
AU  - Yuasa S
FAU - Nakamura, Yasuko
AU  - Nakamura Y
FAU - Suzuki, Eri
AU  - Suzuki E
FAU - Aoki, Masashi
AU  - Aoki M
FAU - Warita, Hitoshi
AU  - Warita H
FAU - Itoyama, Yasuto
AU  - Itoyama Y
FAU - Uchino, Shigeo
AU  - Uchino S
FAU - Kohsaka, Shinichi
AU  - Kohsaka S
FAU - Ohsawa, Keiko
AU  - Ohsawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (SOD1 protein, human)
RN  - EC 1.15.1.1 (Sod1 protein, rat)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
SB  - IM
MH  - Amyotrophic Lateral Sclerosis/enzymology/genetics/*pathology
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Gliosis/enzymology/genetics/pathology
MH  - Humans
MH  - Male
MH  - Microglia/enzymology/*pathology
MH  - Motor Neurons/enzymology/*pathology
MH  - Neurodegenerative Diseases/enzymology/genetics/pathology
MH  - Phagocytosis/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Transgenic
MH  - Spinal Cord/enzymology/*pathology
MH  - Superoxide Dismutase/genetics
MH  - Superoxide Dismutase-1
EDAT- 2010/07/22 06:00
MHDA- 2011/03/12 06:00
CRDT- 2010/07/22 06:00
PHST- 2010/07/22 06:00 [entrez]
PHST- 2010/07/22 06:00 [pubmed]
PHST- 2011/03/12 06:00 [medline]
AID - 10.1002/jnr.22424 [doi]
PST - ppublish
SO  - J Neurosci Res. 2010 Sep;88(12):2736-46. doi: 10.1002/jnr.22424.