PMID- 20649750
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20220408
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 24
IP  - 4
DP  - 2010 Jul-Aug
TI  - Proinflammatory cytokine and chemokine gene expression profiles in subcutaneous 
      and visceral adipose tissue depots of insulin-resistant and insulin-sensitive 
      light breed horses.
PG  - 932-9
LID - 10.1111/j.1939-1676.2010.0551.x [doi]
AB  - BACKGROUND: Insulin resistance has been associated with risk of laminitis in 
      horses. Genes coding for proinflammatory cytokines and chemokines are expressed 
      more in visceral adipose tissue than in subcutaneous adipose tissue of 
      insulin-resistant (IR) humans and rodents. HYPOTHESIS/OBJECTIVES: To investigate 
      adipose depot-specific cytokine and chemokine gene expression in horses and its 
      relationship to insulin sensitivity (SI). ANIMALS: Eleven light breed mares. 
      METHODS: Animals were classified as IR (SI=0.58+/-0.31x10(-4) L/min/mU; n=5) or 
      insulin sensitive (IS; SI=2.59+/-1.21x10(-4) L/min/mU; n=6) based on results of a 
      frequently sampled intravenous glucose tolerance test. Omental, retroperitoneal, 
      and mesocolonic fat was collected by ventral midline celiotomy; incisional nuchal 
      ligament and tail head adipose tissue biopsy specimens were collected 
      concurrently. The expression of tumor necrosis factor-alpha (TNF-alpha), 
      interleukin (IL)-1beta, IL-6, plasminogen activator inhibitor-1 (PAI-1), and 
      monocyte chemoattractant protein-1 (MCP-1) in each depot was measured by 
      real-time quantitative polymerase chain reaction. Data were analyzed by 2-way 
      analysis of variance for repeated measures (P<.05). RESULTS: No differences in 
      TNF-alpha, IL-1beta, IL-6, PAI-1, or MCP-1 mRNA concentrations were noted between 
      IR and IS groups for each depot. Concentrations of mRNA coding for IL-1beta 
      (P=.0005) and IL-6 (P=.004) were significantly higher in nuchal ligament adipose 
      tissue than in other depots. CONCLUSIONS AND CLINICAL IMPORTANCE: These data 
      suggest that the nuchal ligament depot has unique biological behavior in the 
      horse and is more likely to adopt an inflammatory phenotype than other depots 
      examined. Visceral fat may not contribute to the pathogenesis of obesity-related 
      disorders in the horse as in other species.
FAU - Burns, T A
AU  - Burns TA
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The 
      Ohio State University, Columbus, OH 43210, USA.
FAU - Geor, R J
AU  - Geor RJ
FAU - Mudge, M C
AU  - Mudge MC
FAU - McCutcheon, L J
AU  - McCutcheon LJ
FAU - Hinchcliff, K W
AU  - Hinchcliff KW
FAU - Belknap, J K
AU  - Belknap JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Cytokines)
RN  - 0 (Insulin)
SB  - IM
MH  - Adipose Tissue/drug effects/*metabolism
MH  - Animals
MH  - Cytokines/genetics/*metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/physiology
MH  - Horses/*genetics/*physiology
MH  - Insulin/metabolism/*pharmacology
MH  - Insulin Resistance/*genetics
EDAT- 2010/07/24 06:00
MHDA- 2010/09/14 06:00
CRDT- 2010/07/24 06:00
PHST- 2010/07/24 06:00 [entrez]
PHST- 2010/07/24 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
AID - JVIM551 [pii]
AID - 10.1111/j.1939-1676.2010.0551.x [doi]
PST - ppublish
SO  - J Vet Intern Med. 2010 Jul-Aug;24(4):932-9. doi: 10.1111/j.1939-1676.2010.0551.x.