PMID- 2065024
OWN - NLM
STAT- MEDLINE
DCOM- 19910815
LR  - 20071114
IS  - 1043-4674 (Print)
IS  - 1043-4674 (Linking)
VI  - 3
IP  - 4
DP  - 1991 Apr
TI  - Rex-dependent nucleolar accumulation of HTLV-I mRNAs.
PG  - 389-97
AB  - The regulator of virion protein expression (Rex) of the human T-cell leukemia 
      virus type I (HTLV-I) modulates the pattern and level of spliced and unspliced 
      retroviral mRNA species in the cytoplasm. The elucidation of the molecular basis 
      for this regulation is likely to throw light on new regulatory mechanisms of the 
      host cell. We have investigated the possibility that Rex acts to direct unspliced 
      viral RNA to the nucleolus. To test this possibility, we used in situ 
      hybridization to detect HTLV-I envelope protein (env) transcripts formed in the 
      nucleolus in the presence and absence of the Rex protein. The HTLV-I env 
      sequences are normally removed by splicing from the HTLV-I RNA in the absence of 
      Rex. The experiments presented here show that the accumulation of HTLV-I env 
      sequences in the nucleolus is significantly increased in the presence of Rex. The 
      results raise the possibility that Rex directs unspliced viral mRNA to the 
      nucleolus whence it is exported to the cytoplasm for translation. The association 
      between the nucleolus and retroviral mRNA was lost in purified nucleoli after 
      cellular fractionation.
FAU - Kalland, K H
AU  - Kalland KH
AD  - Division of Human Retrovirology, Dana Farber Cancer Institute, Boston, MA 02115.
FAU - Langhoff, E
AU  - Langhoff E
FAU - Bos, H J
AU  - Bos HJ
FAU - Gottlinger, H
AU  - Gottlinger H
FAU - Haseltine, W A
AU  - Haseltine WA
LA  - eng
GR  - CA36974/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - New Biol
JT  - The New biologist
JID - 9000976
RN  - 0 (Gene Products, env)
RN  - 0 (Gene Products, rex)
RN  - 0 (RNA, Messenger)
SB  - IM
GS  - Rex
GS  - env
MH  - Cell Line
MH  - Cell Nucleolus/*metabolism
MH  - Cloning, Molecular
MH  - Cytoplasm/metabolism
MH  - *Gene Expression Regulation, Viral
MH  - Gene Products, env/metabolism
MH  - Gene Products, rex/*physiology
MH  - Human T-lymphotropic virus 1/*genetics/metabolism
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger/*biosynthesis
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PST - ppublish
SO  - New Biol. 1991 Apr;3(4):389-97.