PMID- 20650252
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190918
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 10
IP  - 6
DP  - 1996 Dec
TI  - Comparison of DNA adduct induction in vitro by PAHs and nitro-pahs in freshly 
      isolated rat hepatocytes.
PG  - 685-92
AB  - Polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs have been identified 
      widely in occupational and environmental pollution, such as diesel engine 
      emissions and other combustion products. In most cases, hepatic biotransformation 
      is involved in converting these chemicals to their carcinogenic metabolites. It 
      has been demonstrated that isolated hepatocytes possess substantial amounts of 
      the enzymes responsible for metabolizing xenobiotics and are therefore a 
      convenient model for studying chemicals that require activation to exert their 
      carcinogenic effects. In this study, rat hepatocytes were isolated by collagenase 
      digestion and then exposed to benzo[a]pyrene (B) [a]P), benzo[a]anthracene 
      (B[a]A), 1-nitropyrene (1-NP) and 1,6-dinitropyrene (1,6-DNP) at different doses 
      and/or times so that DNA adducts levels, as measured with the (32)P-postlabelling 
      technique, could be compared. Each of the four compounds tested induced 
      significant increases of total DNA adducts with clear dose-related responses. One 
      or more individual adducts were identified as major adducts for each compound. 
      Time-related increases of DNA adducts were also observed from 1 to 4 hr of 
      incubation. Greater amounts of DNA adducts were induced by B[a]P or 1,6-DNP than 
      by B[a]A or 1-NP, with potency being in the order 1,6-DNP > B[a]P > 1-NP B[a]A. 
      These results demonstrate that freshly isolated hepatocytes can be used as an 
      effective in vitro system for the detection of DNA adducts using 
      (32)P-postlabelling, and have shown 1,6-DNP to be the most potent of the tested 
      constituents of diesel emissions.
FAU - Qu, S X
AU  - Qu SX
AD  - Toxicology Unit, Research, Science and Statistics Division, National Institute of 
      Occupational Health and Safety, Australia.
FAU - Stacey, N H
AU  - Stacey NH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 2010/07/24 06:00
PHST- 1996/09/02 00:00 [accepted]
PHST- 2010/07/24 06:00 [entrez]
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
AID - S0887-2333(96)00062-8 [pii]
AID - 10.1016/s0887-2333(96)00062-8 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 1996 Dec;10(6):685-92. doi: 10.1016/s0887-2333(96)00062-8.