PMID- 20654728
OWN - NLM
STAT- MEDLINE
DCOM- 20110309
LR  - 20191210
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 42
IP  - 10
DP  - 2010 Oct
TI  - Oxidative phosphorylation is impaired by prolonged hypoxia in breast and possibly 
      in cervix carcinoma.
PG  - 1744-51
LID - 10.1016/j.biocel.2010.07.010 [doi]
AB  - It has been assumed that oxidative phosphorylation (OxPhos) in solid tumors is 
      severely reduced due to cytochrome c oxidase substrate restriction, although the 
      measured extracellular oxygen concentration in hypoxic areas seems not limiting 
      for this activity. To identify alternative hypoxia-induced OxPhos depressing 
      mechanisms, an integral analysis of transcription, translation, enzyme activities 
      and pathway fluxes was performed on glycolysis and OxPhos in HeLa and MCF-7 
      carcinomas. In both neoplasias exposed to hypoxia, an early transcriptional 
      response was observed after 8h (two times increased glycolysis-related mRNA 
      synthesis promoted by increased HIF-1alpha levels). However, major metabolic 
      remodeling was observed only after 24h hypoxia: increased glycolytic protein 
      content (1-5-times), enzyme activities (2-times) and fluxes (4-6-times). 
      Interestingly, in MCF-7 cells, 24h hypoxia decreased OxPhos flux (4-6-fold), and 
      2-oxoglutarate dehydrogenase and glutaminase activities (3-fold), with no changes 
      in respiratory complexes I and IV activities. In contrast, 24h hypoxia did not 
      significantly affect HeLa OxPhos flux; neither mitochondria related mRNAs, 
      protein contents or enzyme activities, although the enhanced glycolysis became 
      the main ATP supplier. Thus, prolonged hypoxia (a) targeted some mitochondrial 
      enzymes in MCF-7 but not in HeLa cells, and (b) induced a transition from 
      mitochondrial towards a glycolytic-dependent energy metabolism in both MCF-7 and 
      HeLa carcinomas.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Rodriguez-Enriquez, Sara
AU  - Rodriguez-Enriquez S
AD  - Departamento de Bioquimica, Instituto Nacional de Cardiologia Ignacio Chavez, 
      Mexico DF, Mexico. saren960104@hotmail.com
FAU - Carreno-Fuentes, Liliana
AU  - Carreno-Fuentes L
FAU - Gallardo-Perez, Juan Carlos
AU  - Gallardo-Perez JC
FAU - Saavedra, Emma
AU  - Saavedra E
FAU - Quezada, Hector
AU  - Quezada H
FAU - Vega, Alicia
AU  - Vega A
FAU - Marin-Hernandez, Alvaro
AU  - Marin-Hernandez A
FAU - Olin-Sandoval, Viridiana
AU  - Olin-Sandoval V
FAU - Torres-Marquez, M Eugenia
AU  - Torres-Marquez ME
FAU - Moreno-Sanchez, Rafael
AU  - Moreno-Sanchez R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100721
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - EC 1.2.4.2 (Ketoglutarate Dehydrogenase Complex)
RN  - EC 3.5.1.2 (Glutaminase)
RN  - EC 7.1.1.2 (Electron Transport Complex I)
SB  - IM
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Carcinoma/*metabolism/pathology
MH  - Electron Transport Complex I/metabolism
MH  - Energy Metabolism
MH  - Female
MH  - Glutaminase/genetics/metabolism
MH  - Glycolysis
MH  - HeLa Cells
MH  - Humans
MH  - Hypoxia/*metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis/genetics
MH  - Ketoglutarate Dehydrogenase Complex/genetics/metabolism
MH  - Mitochondria/*metabolism
MH  - Oxidative Phosphorylation
MH  - Uterine Cervical Neoplasms/*metabolism/pathology
EDAT- 2010/07/27 06:00
MHDA- 2011/03/10 06:00
CRDT- 2010/07/27 06:00
PHST- 2010/03/26 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/07/12 00:00 [accepted]
PHST- 2010/07/27 06:00 [entrez]
PHST- 2010/07/27 06:00 [pubmed]
PHST- 2011/03/10 06:00 [medline]
AID - S1357-2725(10)00258-X [pii]
AID - 10.1016/j.biocel.2010.07.010 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2010 Oct;42(10):1744-51. doi: 
      10.1016/j.biocel.2010.07.010. Epub 2010 Jul 21.