PMID- 20655621
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20200930
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 168
IP  - 2
DP  - 2011 Jan 15
TI  - Transgenic carrot tap roots expressing an immunogenic F1-V fusion protein from 
      Yersinia pestis are immunogenic in mice.
PG  - 174-80
LID - 10.1016/j.jplph.2010.06.012 [doi]
AB  - Expression of the protective F1 and V antigens of Yersinia pestis, as a fusion 
      protein, in carrot was pursued in an effort to develop an alternative vaccine 
      production system against the serious plague disease. Transgenic carrot plants 
      carrying the F1-V encoding gene were developed via Agrobacterium-mediated 
      transformation. Presence, integration, and expression of the F1-V encoding gene 
      were confirmed by polymerase chain reaction (PCR), DNA gel blot analysis, and 
      reverse-transcriptase (RT)-PCR analyses, respectively. An ELISA assay confirmed 
      the antigenicity of the plant-derived F1-V fusion protein. Immunogenicity was 
      evaluated subcutaneously in mice using a soluble protein extract of freeze-dried 
      transgenic carrot. Significant antibody levels were detected following 
      immunization. These results demonstrated that the F1-V protein could be expressed 
      in carrot tap roots, and that the carrot F1-V recombinant protein retained its 
      antigenicity and immunogenicity.
CI  - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
FAU - Rosales-Mendoza, Sergio
AU  - Rosales-Mendoza S
AD  - Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, Av. Dr. 
      Manuel Nava 6, Mexico.
FAU - Soria-Guerra, Ruth E
AU  - Soria-Guerra RE
FAU - Moreno-Fierros, Leticia
AU  - Moreno-Fierros L
FAU - Han, Yuepeng
AU  - Han Y
FAU - Alpuche-Solis, Angel G
AU  - Alpuche-Solis AG
FAU - Korban, Schuyler S
AU  - Korban SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100723
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (LcrV protein, Yersinia)
RN  - 0 (Plague Vaccine)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (caf1 protein, Yersinia pestis)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/genetics/*metabolism
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Daucus carota/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plague/immunology
MH  - Plague Vaccine/genetics/immunology/metabolism
MH  - Plant Roots/genetics/*metabolism
MH  - Plants, Genetically Modified/genetics/*metabolism
MH  - Pore Forming Cytotoxic Proteins/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Yersinia pestis/genetics/*metabolism
EDAT- 2010/07/27 06:00
MHDA- 2011/04/08 06:00
CRDT- 2010/07/27 06:00
PHST- 2010/04/25 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/06/19 00:00 [accepted]
PHST- 2010/07/27 06:00 [entrez]
PHST- 2010/07/27 06:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
AID - S0176-1617(10)00275-0 [pii]
AID - 10.1016/j.jplph.2010.06.012 [doi]
PST - ppublish
SO  - J Plant Physiol. 2011 Jan 15;168(2):174-80. doi: 10.1016/j.jplph.2010.06.012. 
      Epub 2010 Jul 23.