PMID- 20660069
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20131121
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 151
IP  - 9
DP  - 2010 Sep
TI  - Identification of early response genes and pathway activated by androgens in the 
      initial segment and caput regions of the regressed rat epididymis.
PG  - 4504-14
LID - 10.1210/en.2010-0023 [doi]
AB  - To identify the initial response to androgens and estrogens in the 
      orchidectomized, regressed epididymis, we determined the gene expression changes 
      triggered by the administration of either of two metabolites of testosterone, 
      5alpha-dihydrotestosterone (DHT) or 17beta-estradiol (E2), in the regressed rat 
      epididymis. Adult rats were orchidectomized and 8 d later implanted with either 
      empty implants (control), DHT-filled-, or E2-filled-polydioxanone implants. Rats 
      were euthanized 12 h, 1 d, and 7 d later, and RNA was extracted and probed on 
      Rat230-2.0 Affymetrix arrays. Probe sets that respond to DHT or E2 were 
      identified at early time points; although the expression of some was repressed, 
      the expression of many others was either transiently or chronically elevated. 
      Nerve growth factor receptor (Ngfr) and S100 calcium binding protein G (S100g) 
      were two E2 up-regulated genes detected at 12 h. Among the genes that showed a 
      dramatic early response to DHT were endothelin 1 (Edn1), bone morphogenetic 
      protein 4 (Bmp4), and IGF binding protein 3 (Igfbp3), which were suppressed, and 
      IGF-I (Igf1), which was induced. Genes that were up- or down-regulated by DHT 
      were classified based on biological function. Using PathwayStudio 4.0, we 
      identified genes that were linked and directly influenced either the expression 
      or regulation of one another. Epidermal growth factor and IGF-I play an important 
      role in the pathway due to their function in regulation and expression of many 
      other genes. These results provide novel insights into the impact of androgen 
      action on the expression of genes that are important for epididymal function.
FAU - Hamzeh, Mahsa
AU  - Hamzeh M
AD  - Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade 
      Sir William Osler, Montreal, Quebec, Canada H3G1Y6.
FAU - Robaire, Bernard
AU  - Robaire B
LA  - eng
GR  - MOP-86735/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100721
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (17-Ketosteroids)
RN  - 0 (5-dihydrorubrosterone)
RN  - 0 (Androstanols)
RN  - 0 (Endothelin-1)
RN  - 4TI98Z838E (Estradiol)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - 17-Ketosteroids/*pharmacology
MH  - Androstanols/*pharmacology
MH  - Animals
MH  - Blotting, Western
MH  - Cluster Analysis
MH  - Endothelin-1/genetics/metabolism
MH  - Epididymis/*drug effects/metabolism/pathology
MH  - Estradiol/*pharmacology
MH  - Gene Expression/*drug effects
MH  - Gene Expression Profiling
MH  - Gene Regulatory Networks
MH  - Insulin-Like Growth Factor I/genetics/metabolism
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Orchiectomy
MH  - Rats
MH  - Rats, Inbred BN
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*drug effects/genetics
MH  - Time Factors
EDAT- 2010/07/28 06:00
MHDA- 2010/10/05 06:00
CRDT- 2010/07/28 06:00
PHST- 2010/07/28 06:00 [entrez]
PHST- 2010/07/28 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - en.2010-0023 [pii]
AID - 10.1210/en.2010-0023 [doi]
PST - ppublish
SO  - Endocrinology. 2010 Sep;151(9):4504-14. doi: 10.1210/en.2010-0023. Epub 2010 Jul 
      21.