PMID- 20661707
OWN - NLM
STAT- MEDLINE
DCOM- 20110418
LR  - 20240109
IS  - 1976-3786 (Electronic)
IS  - 0253-6269 (Linking)
VI  - 33
IP  - 7
DP  - 2010 Jul
TI  - The inhibitory effect of turmeric curcuminoids on matrix metalloproteinase-3 
      secretion in human invasive breast carcinoma cells.
PG  - 989-98
LID - 10.1007/s12272-010-0703-6 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) is a key enzyme with important implications in 
      the invasion and metastasis of breast cancer cells. Curcumin (Cur), 
      demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) are major forms of 
      curcuminoids found in turmeric powder with reported anticancer activity. This 
      study focuses on the comparative effect of Cur, DMC and BDMC on the modulation of 
      MMP-3 activity and its secretion in MDA-MB-231 breast cancer cells. MMP-3 levels 
      were determined by casein zymography, ELISA and western blotting. Analysis of 
      MMP-3 expression by casein zymography revealed high expression in MDA-MB-231 
      invasive breast carcinoma cells, but not in MCF-7 non-invasive breast cancer 
      cells. ELISA assays showed MMP-3 levels were significantly decreased in all 
      curcuminoid treatments. Using zymography, treatment with non-toxic doses revealed 
      that every curcuminoid compound except Cur reduced MMP-3 levels. Moreover, the 
      result from western blot analysis confirmed that only DMC and BDMC reduced MMP-3 
      secretion in MDA-MB-231 cells, but Cur did not have any effect. MMP-3 activity 
      revealed that none of the curcuminoids showed significant effects. However, 
      treatment of the cells with Cur, DMC and BDMC exhibited a significant inhibition 
      of cell invasion and motility with DMC and BDMC being more potent. These results 
      suggest that Cur, DMC, and BDMC may be used as MMP-3 inhibitors to modulate MMP-3 
      expression.
FAU - Boonrao, Mathanaporn
AU  - Boonrao M
AD  - Department of Biochemistry, Chiang Mai University, Thailand.
FAU - Yodkeeree, Supachai
AU  - Yodkeeree S
FAU - Ampasavate, Chadarat
AU  - Ampasavate C
FAU - Anuchapreeda, Songyot
AU  - Anuchapreeda S
FAU - Limtrakul, Pornngarm
AU  - Limtrakul P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100727
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Diarylheptanoids)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 2EFO1BP34R (bisdemethoxycurcumin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - IT942ZTH98 (Curcumin)
RN  - W2F8059T80 (demethoxycurcumin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/isolation & 
      purification/pharmacology/*therapeutic use
MH  - Breast Neoplasms/drug therapy/*enzymology/pathology
MH  - Cell Line, Tumor
MH  - *Curcuma
MH  - Curcumin/*analogs & derivatives/isolation & purification/*therapeutic use
MH  - Diarylheptanoids
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 3/metabolism
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Neoplasm Staging
EDAT- 2010/07/28 06:00
MHDA- 2011/04/19 06:00
CRDT- 2010/07/28 06:00
PHST- 2009/07/31 00:00 [received]
PHST- 2010/03/25 00:00 [accepted]
PHST- 2010/01/18 00:00 [revised]
PHST- 2010/07/28 06:00 [entrez]
PHST- 2010/07/28 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - 10.1007/s12272-010-0703-6 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2010 Jul;33(7):989-98. doi: 10.1007/s12272-010-0703-6. Epub 2010 
      Jul 27.