PMID- 20662036
OWN - NLM
STAT- MEDLINE
DCOM- 20101208
LR  - 20100727
IS  - 1096-9101 (Electronic)
IS  - 0196-8092 (Linking)
VI  - 42
IP  - 6
DP  - 2010 Aug
TI  - Single session to infrared low level diode laser on TNF-alpha and IL-6 cytokines 
      release by mononuclear spleen cells in mice: a pilot study.
PG  - 584-8
LID - 10.1002/lsm.20949 [doi]
AB  - BACKGROUND AND OBJECTIVE: The results of low-level infrared laser (LLL) systemic 
      action on inflammatory modulation process, specifically diminishing 
      pro-inflammatory and producing anti-inflammatory cytokines are extremely 
      controversial in the literature. More studies are necessary to clarify the 
      biomodulation process. The main objective was to investigate the effect of a 
      single session of an AsGaAl laser on spleen cells interleukin-6 (IL-6) and tumor 
      necrosis factor - alpha (TNF-alpha) release, in vivo, in mice. STUDY 
      DESIGN/MATERIALS AND METHODS: In a pilot study, 18 isogenic mice were distributed 
      in three groups: control (no surgical procedure, n = 6), sham (surgical procedure 
      with three standard cutaneous incisions, followed by abdominal muscle incision 
      followed by suture, n = 6) and LLL (same procedure followed by a single LLL 
      exposure 12 hours after the procedure, n = 6). The animals in the LLL group 
      received a single infrared continuous laser session (780 nm wavelength, power of 
      20 mW, energy density of 10 J/cm(2)) on three points (20 seconds per point), and 
      final energy of 0.4 J. All animals of the sham and LLL groups were sacrificed 36 
      hours after surgical procedure; the spleen mononuclear cells were isolated and 
      cultivated for 48 hours. The IL-6 and TNF-alpha were measured by the ELISA 
      method. RESULTS: IL-6 and TNF-alpha concentrations released by the mononuclear 
      cells showed significant differences between the control and sham group (P < 
      0.07). However, there were no differences between the control and LLL group and 
      between the sham and LLL groups (P > 0.07). CONCLUSION: The single session of 
      infrared LLL showed a tendency of decreasing the IL-6 and TNF-alpha release by 
      mononuclear spleen cells in mice after application, although there was not a 
      significant difference between the sham and LLL group. Conclusions regarding 
      effectiveness of a single session procedure cannot be made due to the low 
      statistical power of this pilot study.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Fukuda, Thiago Y
AU  - Fukuda TY
AD  - Experimental Surgery, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP 
      04023-062, Brazil. tfukuda10@yahoo.com.br
FAU - Tanji, Maury M
AU  - Tanji MM
FAU - Jesus, Julio F
AU  - Jesus JF
FAU - Sato, Maria N
AU  - Sato MN
FAU - Duarte, Alberto J S
AU  - Duarte AJ
FAU - Plapler, Helio
AU  - Plapler H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lasers Surg Med
JT  - Lasers in surgery and medicine
JID - 8007168
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukin-6/*metabolism
MH  - *Lasers
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/*metabolism
MH  - Pilot Projects
MH  - Spleen/*cytology
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2010/07/28 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/07/28 06:00
PHST- 2010/07/28 06:00 [entrez]
PHST- 2010/07/28 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1002/lsm.20949 [doi]
PST - ppublish
SO  - Lasers Surg Med. 2010 Aug;42(6):584-8. doi: 10.1002/lsm.20949.