PMID- 20662075
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20100920
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 49
IP  - 11
DP  - 2010 Nov
TI  - Interphase fluorescence in situ hybridization on selected plasma cells is 
      superior in the detection of cytogenetic aberrations in plasma cell dyscrasia.
PG  - 991-7
LID - 10.1002/gcc.20809 [doi]
AB  - Interphase fluorescence in situ hybridization (FISH) detects nonrandom 
      cytogenetic abnormalities in plasma cell (PC) dyscrasia according to PC burden. 
      However, when performed on cultured whole bone marrow (BM), it often fails to 
      detect these aberrations. We have compared this interphase FISH technique with 
      FISH after PC purification or identification to detect recurrent aberrations. In 
      this study, 235 BM samples were collected from patients with multiple myeloma 
      (MM) or related PC disorders regardless of disease status. All samples were 
      analyzed in parallel. Clonal abnormalities were detected in 34.9% of cultured 
      samples compared with 71.0% PC selected samples (P < 0.001). Moreover, FISH on 
      PCs allowed to detect more abnormalities per case (P < 0.001) and identified 
      higher percentages of abnormal nuclei (P < 0.001). This study indicates that FISH 
      on PCs is the preferred technique for routine cytogenetic investigation of MM.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Put, Natalie
AU  - Put N
AD  - Centrum voor Menselijke Erfelijkheid, Katholieke Universiteit Leuven, Leuven, 
      Belgium.
FAU - Lemmens, Heidi
AU  - Lemmens H
FAU - Wlodarska, Iwona
AU  - Wlodarska I
FAU - Konings, Peter
AU  - Konings P
FAU - Moreau, Yves
AU  - Moreau Y
FAU - Hagemeijer, Anne
AU  - Hagemeijer A
FAU - Vandenberghe, Peter
AU  - Vandenberghe P
FAU - Michaux, Lucienne
AU  - Michaux L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Male
MH  - Middle Aged
MH  - Paraproteinemias/*genetics
EDAT- 2010/07/28 06:00
MHDA- 2011/01/14 06:00
CRDT- 2010/07/28 06:00
PHST- 2010/07/28 06:00 [entrez]
PHST- 2010/07/28 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
AID - 10.1002/gcc.20809 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2010 Nov;49(11):991-7. doi: 10.1002/gcc.20809.