PMID- 20665654
OWN - NLM
STAT- MEDLINE
DCOM- 20110610
LR  - 20161125
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 4
IP  - 8
DP  - 2010 Dec
TI  - Cyclosporine-impregnated allograft bone sterilized with low-temperature plasma.
PG  - 638-51
LID - 10.1002/term.279 [doi]
AB  - Deep-freezing, freeze-drying and gamma (gamma)-irradiation have deleterious effects 
      on bone healing and mechanical properties of allograft bones. We tried preparing 
      bone allografts using cyclosporine plus low-temperature-plasma sterilization. To 
      explore the feasibility of this method of preparation, segmental defects in the 
      right radii of rabbits were repaired with cyclosporine-impregnated allograft 
      bones (CABs) sterilized with low-temperature-plasma (in the study group) and 
      deep-frozen/freeze-dried irradiated allograft bones (D/FIABs) (in the control 
      group). X-ray and quantitative histological analysis, peripheral blood T 
      lymphocyte subset analysis and CD(2)(5) molecule immunohistochemistry stain, the 
      four-point bending test and safety evaluations were respectively conducted to 
      compare bone-healing, immunosuppression, mechanical properties and safety between 
      the two groups. X-ray scores were higher in the study group than those in the 
      control (p = 0.032). There were significant differences in new bone areas at most 
      repairs in both groups (p < 0.05). There were no significant differences in the 
      percentages of CD(4)(+) T, CD(8)(+) T, ratios of CD(4)(+) T:CD(8)(+) T or serum 
      concentrations of GPT/Cr in both groups (p > 0.05). At 16 weeks postoperatively, 
      the density of CD(2)(5) molecules in the control group was higher than that in the 
      study group. The ultimate loading in the study group was significantly higher 
      than that in the control (p = 0.048). Bone marrow stromal cells (BMSCs) grew 
      thickly around and on the surface of a cyclosporine-impregnated allograft. Livers 
      and kidneys in the study and control groups remained histologically normal at 7 
      days postoperatively. These results indicate that the CAB might be a better 
      material than the D/FIAB in terms of bone healing, preservation of mechanical 
      properties and immunosuppression without severe side-effects.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Lu, Haibo
AU  - Lu H
AD  - Institute of Orthopedics of Chinese PLA, Chinese PLA General Hospital, Beijing 
      100853, People's Republic of China.
FAU - Pei, Guoxian
AU  - Pei G
FAU - Zhao, Peiran
AU  - Zhao P
FAU - Liang, Shuangwu
AU  - Liang S
FAU - Jin, Dan
AU  - Jin D
FAU - Jiang, Shan
AU  - Jiang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Plasma Gases)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects
MH  - *Bone Transplantation
MH  - Bone and Bones/diagnostic imaging/pathology/surgery
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Cyclosporine/*pharmacology
MH  - Immunohistochemistry
MH  - Implants, Experimental
MH  - Kidney/cytology/drug effects
MH  - Liver/cytology/drug effects
MH  - Osteogenesis/drug effects
MH  - Plasma Gases/*pharmacology
MH  - Rabbits
MH  - Radiography
MH  - *Sterilization
MH  - Stromal Cells/cytology/drug effects
MH  - T-Lymphocytes/drug effects/immunology
MH  - *Temperature
MH  - Transplantation, Homologous
MH  - Wound Healing/drug effects
EDAT- 2010/07/29 06:00
MHDA- 2011/06/11 06:00
CRDT- 2010/07/29 06:00
PHST- 2010/07/29 06:00 [entrez]
PHST- 2010/07/29 06:00 [pubmed]
PHST- 2011/06/11 06:00 [medline]
AID - 10.1002/term.279 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2010 Dec;4(8):638-51. doi: 10.1002/term.279.