PMID- 20665655
OWN - NLM
STAT- MEDLINE
DCOM- 20101222
LR  - 20151119
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 118
IP  - 5
DP  - 2010 Oct 25
TI  - Computerized analysis of cytology and fluorescence in situ hybridization (FISH) 
      in induced sputum for lung cancer detection.
PG  - 269-77
LID - 10.1002/cncy.20094 [doi]
AB  - BACKGROUND: Lung cancer results from a multistep process, whereby genetic and 
      epigenetic alterations lead to a malignant phenotype. Somatic mutations, 
      deletions, and amplifications can be detected in the tumor itself, but they can 
      also be found in histologically normal bronchial epithelium as a result of field 
      cancerization. The present feasibility study describes a computer-assisted 
      analysis of induced sputum employing morphology and fluorescence in situ 
      hybridization (target-FISH), using 2 biomarkers located at chromosomes 3p22.1 and 
      10q22.3. METHODS: Induced sputum samples were collected using a standardized 
      protocol from 12 patients with lung cancer and from 15 healthy, nonsmoking 
      controls. We used an automated scanning system that allows consecutive scans of 
      morphology and FISH of the same slide. Cells derived for the lower airways were 
      analyzed for the presence of genetic alterations in the 3p22.1 and 10q22.3 loci. 
      RESULTS: The cutoff for a positive diagnosis was defined as >4% of cells showing 
      genetic alterations. Eleven of 12 lung cancer patients and 12 of 15 controls were 
      identified correctly, giving an overall sensitivity and specificity of 91.66% and 
      80%, respectively. CONCLUSIONS: This study describes a new technology for 
      detecting lung cancer noninvasively in induced sputum via a combination of 
      morphology and FISH analysis (target-FISH) using computer-assisted technology. 
      This approach may potentially be utilized for mass screening of high-risk 
      populations.
CI  - (c) 2010 American Cancer Society.
FAU - Guber, Alexander
AU  - Guber A
AD  - Pulmonary Department, Meir Hospital, Kfar Saba, Israel.
FAU - Greif, Joel
AU  - Greif J
FAU - Rona, Roni
AU  - Rona R
FAU - Fireman, Elizabeth
AU  - Fireman E
FAU - Madi, Lea
AU  - Madi L
FAU - Kaplan, Tal
AU  - Kaplan T
FAU - Yemini, Zipi
AU  - Yemini Z
FAU - Gottfried, Maya
AU  - Gottfried M
FAU - Katz, Ruth L
AU  - Katz RL
FAU - Daniely, Michal
AU  - Daniely M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Pulmonary Surfactant-Associated Protein A)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (SFTPA1 protein, human)
RN  - 0 (SFTPA2 protein, human)
RN  - 0 (ribosomal protein L14)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Chromosomes, Human, Pair 10/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Cytodiagnosis/methods
MH  - Diagnosis, Computer-Assisted/methods
MH  - Feasibility Studies
MH  - Female
MH  - Genetic Testing/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/diagnosis/*genetics
MH  - Male
MH  - Pulmonary Surfactant-Associated Protein A/genetics
MH  - Reproducibility of Results
MH  - Ribosomal Proteins/genetics
MH  - Sensitivity and Specificity
MH  - Sputum/*metabolism
EDAT- 2010/07/29 06:00
MHDA- 2010/12/24 06:00
CRDT- 2010/07/29 06:00
PHST- 2010/07/29 06:00 [entrez]
PHST- 2010/07/29 06:00 [pubmed]
PHST- 2010/12/24 06:00 [medline]
AID - 10.1002/cncy.20094 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2010 Oct 25;118(5):269-77. doi: 10.1002/cncy.20094.