PMID- 20667670
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 147
IP  - 3-4
DP  - 2011 Jan 27
TI  - Complete genomic characterization of a Chinese isolate of porcine reproductive 
      and respiratory syndrome virus.
PG  - 274-82
LID - 10.1016/j.vetmic.2010.07.001 [doi]
AB  - The genome of one isolate of porcine reproductive and respiratory syndrome virus 
      (PRRSV) from China, designated GDQY2, was sequenced and analyzed. The full length 
      of GDQY2 was 15,215 nucleotides, excluding the poly(A) tail. Comparative analysis 
      with the genomic sequences of numerous worldwide North American isolates revealed 
      that GDQY2 shared 85.0-98.9% identity with these isolates, but only 60.9% with 
      the European virus-LV (Lelystad Virus), indicating that this new Chinese isolate 
      was closely related to the North American PRRSV genotype. Phylogenetic analysis 
      based on the nucleotide sequences of the full length and ORF5 showed that this 
      new isolate belong to the same genetic group with all other Chinese isolates. 
      Comparison with North American PRRSV isolates revealed that GDQY2 exhibited 
      variations in the non-structural protein 2 (NSP2) encoded by ORF1a, namely that 
      an additional 35-amino acid deletion in NSP2 was found in GDQY2. Therefore, GDQY2 
      was a novel strain with unique deletions. Furthermore, our study demonstrated 
      that North American genotype PRRSVs in China have evolved independently from 
      other countries, indicating that geographic separation might be one factor 
      influencing the molecular evolution of PRRSV.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Zhu, Ling
AU  - Zhu L
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou 510642, Guangdong, PR China.
FAU - Zhang, Guanqun
AU  - Zhang G
FAU - Ma, Jingyun
AU  - Ma J
FAU - He, Xiaoming
AU  - He X
FAU - Xie, Qingmei
AU  - Xie Q
FAU - Bee, Yingzuo
AU  - Bee Y
FAU - Gong, Shan Zhong
AU  - Gong SZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100707
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Viral/immunology
MH  - China
MH  - Genes, Viral/genetics
MH  - Genome, Viral/*genetics
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - *Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*genetics
MH  - Sequence Alignment
MH  - Swine
EDAT- 2010/07/30 06:00
MHDA- 2011/06/24 06:00
CRDT- 2010/07/30 06:00
PHST- 2010/02/04 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/07/01 00:00 [accepted]
PHST- 2010/07/30 06:00 [entrez]
PHST- 2010/07/30 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
AID - S0378-1135(10)00336-6 [pii]
AID - 10.1016/j.vetmic.2010.07.001 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 Jan 27;147(3-4):274-82. doi: 10.1016/j.vetmic.2010.07.001. 
      Epub 2010 Jul 7.