PMID- 20668576 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20110714 LR - 20211020 IS - 0976-4062 (Electronic) IS - 0973-1296 (Print) IS - 0973-1296 (Linking) VI - 6 IP - 22 DP - 2010 Apr TI - Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD. PG - 111-5 LID - 10.4103/0973-1296.62896 [doi] AB - A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R(2) > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 mug/ml and 0.13 to 1.11 mug/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%. FAU - Won, Jin Bae AU - Won JB AD - Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea. FAU - Ma, Jin Yeul AU - Ma JY FAU - Um, Young Ran AU - Um YR FAU - Ma, Choong Je AU - Ma CJ LA - eng PT - Journal Article DEP - 20100505 PL - United States TA - Pharmacogn Mag JT - Pharmacognosy magazine JID - 101300403 PMC - PMC2900057 OTO - NOTNLM OT - HPLC- DAD OT - Herbal medicine OT - Ssanghwa tang OT - quality control OT - quantification COIS- Conflict of Interest: None declared EDAT- 2010/07/30 06:00 MHDA- 2010/07/30 06:01 PMCR- 2010/04/01 CRDT- 2010/07/30 06:00 PHST- 2010/01/12 00:00 [received] PHST- 2010/02/22 00:00 [revised] PHST- 2010/05/05 00:00 [accepted] PHST- 2010/07/30 06:00 [entrez] PHST- 2010/07/30 06:00 [pubmed] PHST- 2010/07/30 06:01 [medline] PHST- 2010/04/01 00:00 [pmc-release] AID - PM-06-111 [pii] AID - 10.4103/0973-1296.62896 [doi] PST - ppublish SO - Pharmacogn Mag. 2010 Apr;6(22):111-5. doi: 10.4103/0973-1296.62896. Epub 2010 May 5.