PMID- 20670450
OWN - NLM
STAT- MEDLINE
DCOM- 20100916
LR  - 20211203
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 11
DP  - 2010 Jul 30
TI  - Microarray analysis of response of Salmonella during infection of HLA-B27- 
      transfected human macrophage-like U937 cells.
PG  - 456
LID - 10.1186/1471-2164-11-456 [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA)-B27 is strongly associated with the 
      development of reactive arthritis (ReA) in humans after salmonellosis. Human 
      monocytic U937 cells transfected with HLA-B27 are less able to eliminate 
      intracellular Salmonella enterica serovar Enteritidis than those transfected with 
      control HLA antigens (e.g. HLA-A2). To investigate further the mechanisms by 
      which HLA-B27-transfected cells allow increased replication of these bacteria, a 
      DNA-based microarray was used for comparative genomic analysis of S. Enteritidis 
      grown in HLA-B27- or HLA-A2-transfected cells. The microarray consisted of 5080 
      oligonucleotides from different serovars of Salmonella including S. Enteritidis 
      PT4-specific genes. Bacterial RNA was isolated from the infected HLA-B27- or 
      HLA-A2-transfected cells, reverse-transcribed to cDNA, and hybridized with the 
      oligonucleotides on the microarrays. Some microarray results were confirmed by 
      RT-PCR. RESULTS: When gene expression was compared between Salmonella grown in 
      HLA-B27 cells and in HLA-A2 cells, 118 of the 4610 S. Enteritidis-related genes 
      differed in expression at 8 h after infection, but no significant difference was 
      detectable at 2 h after infection. These differentially expressed genes are 
      mainly involved in Salmonella virulence, DNA replication, energy conversion and 
      metabolism, and uptake and metabolism of nutrient substances, etc. The difference 
      suggests HLA-B27-dependent modulation of Salmonella gene expression, resulting in 
      increased Salmonella replication in HLA-B27-positive cells. Among the 
      up-regulated genes were those located in Salmonella pathogenicity island (SPI)-2, 
      which play a central role in intracellular survival and replication of 
      Salmonella. CONCLUSIONS: This is the first report to show the regulation of 
      Salmonella gene expression by HLA-B27 during infection of host cells. This 
      regulation probably leads to increased Salmonella survival and replication in 
      HLA-B27-positive cells. SPI-2 genes seem to contribute significantly to the 
      increased replication.
FAU - Ge, Shichao
AU  - Ge S
AD  - Department of Infectious Disease Surveillance and Control, National Institute for 
      Health and Welfare, Turku, Finland.
FAU - Danino, Vittoria
AU  - Danino V
FAU - He, Qiushui
AU  - He Q
FAU - Hinton, Jay C D
AU  - Hinton JC
FAU - Granfors, Kaisa
AU  - Granfors K
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100730
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (DNA, Bacterial)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - DNA Replication
MH  - DNA, Bacterial/genetics/metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - HLA-B27 Antigen/*immunology
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis
MH  - Prohibitins
MH  - Reactive Oxygen Species/metabolism
MH  - Salmonella/*genetics/physiology
MH  - U937 Cells
PMC - PMC3091652
EDAT- 2010/07/31 06:00
MHDA- 2010/09/18 06:00
PMCR- 2010/07/30
CRDT- 2010/07/31 06:00
PHST- 2009/12/03 00:00 [received]
PHST- 2010/07/30 00:00 [accepted]
PHST- 2010/07/31 06:00 [entrez]
PHST- 2010/07/31 06:00 [pubmed]
PHST- 2010/09/18 06:00 [medline]
PHST- 2010/07/30 00:00 [pmc-release]
AID - 1471-2164-11-456 [pii]
AID - 10.1186/1471-2164-11-456 [doi]
PST - epublish
SO  - BMC Genomics. 2010 Jul 30;11:456. doi: 10.1186/1471-2164-11-456.