PMID- 20670477
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20151119
IS  - 1607-8454 (Electronic)
IS  - 1024-5332 (Linking)
VI  - 15
IP  - 4
DP  - 2010 Aug
TI  - Identification of new markers discriminating between myeloid and lymphoid acute 
      leukemia.
PG  - 193-203
LID - 10.1179/102453310X12647083620769 [doi]
AB  - BACKGROUND: The heterogeneity of acute myeloid leukemia (AML) with respect to 
      biology and clinical course resides in the fact that patients belonging to the 
      same group show marked differences in their response to chemotherapy, 
      necessitating a refinement of AML classification. METHODS: In order to define 
      molecular markers for AML, we performed microarray analysis on peripheral blood 
      cells from two M5 AML patients, and selected four differentially expressed genes 
      to validate their expression by real-time quantitative PCR (RT-PCR). RESULTS: We 
      have shown that two downregulated genes in AML, those encoding guanine 
      nucleotide-binding protein gamma11 (GNG11) and amphiregulin (AREG), are also 
      downregulated in B-lineage acute lymphoblastic leukemia (B-ALL) and T-lineage 
      acute lymphoblastic leukemia (T-ALL) patients. A second gene, that encoding 
      ceruloplasmin (CP), is upregulated in AML but not in B-ALL and T-ALL. The level 
      of expression of these genes varies from one patient to another. CONCLUSION: 
      Since the number of patients studied is limited, further studies are needed with 
      a larger series of patients to evaluate the potential utility of GNG11, AREG and 
      CP as molecular markers for AML subtype classification. Our study is the first to 
      analyze these genes in AML, B-ALL, T-ALL and chronic leukemia (myeloid and 
      lymphoid) patients by RT-PCR. This rapid and sensitive method could be used to 
      screen these genes in different types of leukemia.
FAU - Haouas, Houda
AU  - Haouas H
AD  - Department of Biological and Chemical Engineering, National Institute of Applied 
      Sciences and Technology, Tunis, Tunisia. haouas_h@yahoo.com
FAU - Haouas, Samira
AU  - Haouas S
FAU - Uzan, Georges
AU  - Uzan G
FAU - Hafsia, Aicha
AU  - Hafsia A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - EC 1.16.3.1 (Ceruloplasmin)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gq-G11)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Amphiregulin
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Ceruloplasmin/genetics/metabolism
MH  - Diagnosis, Differential
MH  - EGF Family of Proteins
MH  - Female
MH  - GTP-Binding Protein alpha Subunits, Gq-G11/genetics/metabolism
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Leukemic
MH  - Glycoproteins/genetics/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Leukemia, Myeloid, Acute/blood/*diagnosis/*genetics
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/blood/diagnosis/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood/*diagnosis/*genetics
MH  - Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/blood/diagnosis/genetics
EDAT- 2010/07/31 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/07/31 06:00
PHST- 2010/07/31 06:00 [entrez]
PHST- 2010/07/31 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - 10.1179/102453310X12647083620769 [doi]
PST - ppublish
SO  - Hematology. 2010 Aug;15(4):193-203. doi: 10.1179/102453310X12647083620769.