PMID- 20670623
OWN - NLM
STAT- MEDLINE
DCOM- 20101215
LR  - 20131121
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 89
IP  - 3
DP  - 2010 Dec
TI  - Protective effects of melatonin on testicular torsion/detorsion-induced 
      ischemia-reperfusion injury in rats.
PG  - 314-20
LID - 10.1016/j.yexmp.2010.07.006 [doi]
AB  - Testicular torsion causes an enhanced formation of reactive oxygen species, which 
      contributes to the pathophysiology of tissue damage. The aim of this study was to 
      investigate the protective effect of melatonin on testicular 
      torsion/detorsion-induced ischemia-reperfusion (I/R) injury. A total of 24 male 
      Wistar albino rats were divided into three groups of 8 animals each: control, 
      I/R, and I/R treated with melatonin. The ischemia period was 5 h and orchiectomy 
      was performed after 5 h of detorsion. Melatonin (10 mg/kg, intraperitoneally 
      [i.p.]) was administrated only once, 40 min prior to detorsion. Spermatogenesis 
      and mean seminiferous tubule diameter (MSTD) were significantly decreased in the 
      I/R groups were compared to the control group. Furthermore, the melatonin treated 
      animals showed an improved histological appearance in the I/R group. Our data 
      indicate a significant reduction in the activity of TUNEL; there was a rise in 
      the expression of proliferating cell nuclear antigen (PCNA) and testosterone in 
      testes tissue of the I/R group treated with melatonin therapy. Electron 
      microscopy of the testes of the rats demonstrated that pretreatment with 
      melatonin was particularly effective in preventing mitochondrial degeneration, 
      dilatation of SER, and enlarged intercellular spaces in both Sertoli and 
      spermatid cells in the I/R treated animals. We believe that further preclinical 
      research into the utility of melatonin may indicate its usefulness as a potential 
      treatment on testes injury after I/R in rats.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Kanter, M
AU  - Kanter M
AD  - Department of Histology and Embryology, Faculty of Medicine, University of 
      Trakya, Edirne 22030, Turkey. mkanter65@yahoo.com
LA  - eng
PT  - Journal Article
DEP - 20100727
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Antioxidants)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/drug effects
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Microscopy, Electron, Transmission
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/etiology/pathology/*prevention & control
MH  - Spermatic Cord Torsion/complications/*drug therapy/pathology
MH  - Spermatozoa/drug effects/pathology
EDAT- 2010/07/31 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/07/31 06:00
PHST- 2010/02/03 00:00 [received]
PHST- 2010/07/19 00:00 [revised]
PHST- 2010/07/19 00:00 [accepted]
PHST- 2010/07/31 06:00 [entrez]
PHST- 2010/07/31 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - S0014-4800(10)00103-6 [pii]
AID - 10.1016/j.yexmp.2010.07.006 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2010 Dec;89(3):314-20. doi: 10.1016/j.yexmp.2010.07.006. Epub 
      2010 Jul 27.