PMID- 20670665
OWN - NLM
STAT- MEDLINE
DCOM- 20110225
LR  - 20220318
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 154
IP  - 1-2
DP  - 2010 Dec
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral 
      fluid samples from individual boars: will oral fluid replace serum for PRRSV 
      surveillance?
PG  - 170-6
LID - 10.1016/j.virusres.2010.07.025 [doi]
AB  - The purpose of this study was to determine whether oral fluid samples could be 
      used to monitor individually-housed adult boars for porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection. In 3 trials, 24 boars were 
      intramuscularly (IM) inoculated with a modified-live PRRSV (MLV) vaccine (Trial 
      1), a Type 1 PRRSV isolate (Trial 2), or a Type 2 isolate (Trial 3). Oral fluid 
      samples were collected daily and serum samples were collected twice weekly. 
      Following the completion of the study, samples were randomized and blind-tested 
      for PRRSV by real-time quantitative reverse-transcription polymerase chain 
      reaction (qRT-PCR). PRRSV was detected in oral fluids at DPI 1 and all oral fluid 
      specimens were PRRSV qRT-PCR positive at DPI 4. Although PRRSV was detected in 
      both serum and oral fluid specimens through DPI 21, a comparison of matched 
      samples from individual boars showed that oral fluid was equal to serum for the 
      detection of PRRSV at DPI 7 and more likely to be positive than serum on DPI 14 
      and 21. Overall, oral fluid was superior to serum for the detection of PRRSV 
      using PCR over the 21-day observation period in this study. The results of this 
      experiment suggest that individually-penned oral fluid sampling could be an 
      efficient, cost-effective approach to PRRSV surveillance in boar studs and other 
      swine populations.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Kittawornrat, Apisit
AU  - Kittawornrat A
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. 
      apisitk@iastate.edu
FAU - Prickett, John
AU  - Prickett J
FAU - Chittick, Wayne
AU  - Chittick W
FAU - Wang, Chong
AU  - Wang C
FAU - Engle, Mark
AU  - Engle M
FAU - Johnson, Jeremy
AU  - Johnson J
FAU - Patnayak, Devi
AU  - Patnayak D
FAU - Schwartz, Trevor
AU  - Schwartz T
FAU - Whitney, Daniel
AU  - Whitney D
FAU - Olsen, Chris
AU  - Olsen C
FAU - Schwartz, Kent
AU  - Schwartz K
FAU - Zimmerman, Jeffrey
AU  - Zimmerman J
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
DEP - 20100727
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - RNA, Viral/genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Saliva/*virology
MH  - Serum/*virology
MH  - Swine
MH  - Viral Load
MH  - Virology/methods
EDAT- 2010/07/31 06:00
MHDA- 2011/02/26 06:00
CRDT- 2010/07/31 06:00
PHST- 2010/04/12 00:00 [received]
PHST- 2010/07/10 00:00 [revised]
PHST- 2010/07/20 00:00 [accepted]
PHST- 2010/07/31 06:00 [entrez]
PHST- 2010/07/31 06:00 [pubmed]
PHST- 2011/02/26 06:00 [medline]
AID - S0168-1702(10)00266-2 [pii]
AID - 10.1016/j.virusres.2010.07.025 [doi]
PST - ppublish
SO  - Virus Res. 2010 Dec;154(1-2):170-6. doi: 10.1016/j.virusres.2010.07.025. Epub 
      2010 Jul 27.