PMID- 20673022
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20220330
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 16
IP  - 12
DP  - 2010 Dec
TI  - Chondrogenesis and mineralization during in vitro culture of human mesenchymal 
      stem cells on three-dimensional woven scaffolds.
PG  - 3709-18
LID - 10.1089/ten.TEA.2010.0190 [doi]
AB  - Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven 
      poly(varepsilon-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue 
      engineering. We hypothesized that in vitro culture duration and medium additives 
      can individually and interactively influence the structure, composition, 
      mechanical, and molecular properties of engineered tissues based on hMSCs and 3D 
      poly(varepsilon-caprolactone). Bone marrow hMSCs were suspended in collagen gel, seeded on 
      scaffolds, and cultured for 1, 21, or 45 days under chondrogenic and/or 
      osteogenic conditions. Structure, composition, biomechanics, and gene expression 
      were analyzed. In chondrogenic medium, cartilaginous tissue formed by day 21, and 
      hypertrophic mineralization was observed in the newly formed extracellular matrix 
      at the interface with underlying scaffold by day 45. Glycosaminoglycan, 
      hydroxyproline, and calcium contents, and alkaline phosphatase activity depended 
      on culture duration and medium additives, with significant interactive effects 
      (all p < 0.0001). The 45-day constructs exhibited mechanical properties on the 
      order of magnitude of native articular cartilage (aggregate, Young's, and shear 
      moduli of 0.15, 0.12, and 0.033 MPa, respectively). Gene expression was 
      characteristic of chondrogenesis and endochondral bone formation, with sequential 
      regulation of Sox-9, collagen type II, aggrecan, core binding factor alpha 1 
      (Cbfalpha1)/Runx2, bone sialoprotein, bone morphogenetic protein-2, and osteocalcin. 
      In contrast, osteogenic medium produced limited osteogenesis. Long-term culture 
      of hMSC on 3D scaffolds resulted in chondrogenesis and regional mineralization at 
      the interface between soft, newly formed engineered cartilage, and stiffer 
      underlying scaffold. These findings merit consideration when developing grafts 
      for osteochondral defect repair.
FAU - Abrahamsson, Christoffer K
AU  - Abrahamsson CK
AD  - Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute 
      of Technology, Cambridge, Massachusetts, USA.
FAU - Yang, Fan
AU  - Yang F
FAU - Park, Hyoungshin
AU  - Park H
FAU - Brunger, Jonathan M
AU  - Brunger JM
FAU - Valonen, Piia K
AU  - Valonen PK
FAU - Langer, Robert
AU  - Langer R
FAU - Welter, Jean F
AU  - Welter JF
FAU - Caplan, Arnold I
AU  - Caplan AI
FAU - Guilak, Farshid
AU  - Guilak F
FAU - Freed, Lisa E
AU  - Freed LE
LA  - eng
GR  - AR057600/AR/NIAMS NIH HHS/United States
GR  - AR055042/AR/NIAMS NIH HHS/United States
GR  - AR055414/AR/NIAMS NIH HHS/United States
GR  - R01 AR050208-05/AR/NIAMS NIH HHS/United States
GR  - DE016516/DE/NIDCR NIH HHS/United States
GR  - AR050208/AR/NIAMS NIH HHS/United States
GR  - R01 AR050208/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100906
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
SB  - IM
MH  - Cells, Cultured
MH  - Chondrogenesis/*physiology
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Middle Aged
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
PMC - PMC2991213
EDAT- 2010/08/03 06:00
MHDA- 2011/04/08 06:00
PMCR- 2011/12/01
CRDT- 2010/08/03 06:00
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2010.0190 [pii]
AID - 10.1089/ten.TEA.2010.0190 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2010 Dec;16(12):3709-18. doi: 10.1089/ten.TEA.2010.0190. Epub 
      2010 Sep 6.