PMID- 20673108
OWN - NLM
STAT- MEDLINE
DCOM- 20100914
LR  - 20100802
IS  - 0003-1488 (Print)
IS  - 0003-1488 (Linking)
VI  - 237
IP  - 3
DP  - 2010 Aug 1
TI  - Comparison of complete blood counts in samples obtained from healthy dogs and 
      cats by use of standard and microsample blood collection tubes.
PG  - 281-7
LID - 10.2460/javma.237.3.281 [doi]
AB  - OBJECTIVE: To compare results of a CBC performed on blood samples obtained from 
      healthy dogs and cats by use of standard and microsample collection tubes. 
      DESIGN: Evaluation study. ANIMALS: 29 healthy client-owned animals (14 dogs and 
      15 cats). PROCEDURES: A blood sample (3 mL) was collected from each animal; 2.5 
      mL was transferred into a vacuum tube that contained sodium EDTA, and 0.5 mL was 
      transferred into a microsample tube that contained sodium EDTA. Variables 
      evaluated were total numbers of RBCs and WBCs, hemoglobin concentration, Hct, 
      mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin 
      concentration (MCHC), mean platelet volume, and plasma total protein 
      concentration as well as neutrophil, lymphocyte, monocyte, eosinophil, basophil, 
      and platelet counts. Results for the 2 types of tube in each species were 
      compared by use of Pearson correlation coefficients, Passing-Bablok regression 
      analysis, and Bland-Altman analysis. RESULTS: The Pearson correlation coefficient 
      was low for basophil count in cats and moderate, high, or very high for all other 
      variables. Constant and proportional biases were identified for MCHC in dogs by 
      use of Passing-Bablok regression analysis, although the mean difference between 
      types of blood collection tubes was small. No evidence of constant or 
      proportional bias for any other variable was revealed by regression analysis or 
      Bland-Altman analysis. CONCLUSIONS AND CLINICAL RELEVANCE: Samples obtained from 
      healthy dogs and cats by use of microsample blood collection tubes provided 
      clinically equivalent CBC results, compared with results for samples obtained by 
      use of standard blood collection tubes, and minimized the total sample volume 
      collected for diagnostic testing.
FAU - Whittemore, Jacqueline C
AU  - Whittemore JC
AD  - Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 
      University of Tennessee, Knoxville, TN 37996, USA. jwhittemore@utk.edu
FAU - Flatland, Bente
AU  - Flatland B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Vet Med Assoc
JT  - Journal of the American Veterinary Medical Association
JID - 7503067
SB  - IM
MH  - Animals
MH  - Blood Cell Count/veterinary
MH  - Blood Specimen Collection/instrumentation/methods/*veterinary
MH  - Cats/*blood
MH  - Dogs/*blood
MH  - Female
MH  - Male
EDAT- 2010/08/03 06:00
MHDA- 2010/09/16 06:00
CRDT- 2010/08/03 06:00
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2010/09/16 06:00 [medline]
AID - 10.2460/javma.237.3.281 [doi]
PST - ppublish
SO  - J Am Vet Med Assoc. 2010 Aug 1;237(3):281-7. doi: 10.2460/javma.237.3.281.