PMID- 20673302
OWN - NLM
STAT- MEDLINE
DCOM- 20110126
LR  - 20111117
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 22
IP  - 10
DP  - 2010 Oct
TI  - Mapping of kisspeptin fibres in the brain of the pro-oestrous rat.
PG  - 1101-12
LID - 10.1111/j.1365-2826.2010.02053.x [doi]
AB  - Kisspeptins are a family of small peptides that play a key role in the 
      neuroendocrine regulation of the reproductive function through neural pathways 
      that have not yet been completely identified. The present study aimed to 
      investigate the distribution of kisspeptin neurone fibres in the female rat brain 
      by comparing precisely the immunoreactive pattern obtained with two antibodies: 
      one specifically directed against kisspeptin-52 (Kp-52), the longest isoform, and 
      the other directed against kisspeptin-10 (Kp-10), whose sequence is common to all 
      putative mature isoforms. With both antibodies, immunoreactive cell bodies were 
      exclusively observed in the arcuate nucleus, and immunoreactive fibres were 
      confined to the septo-preoptico-hypothalamic continuum of the brain. Fibres were 
      observed in the preoptic area, the diagonal band of Broca, the septohypothalamic 
      area, the anteroventral periventricular, suprachiasmatic, supraoptic, 
      paraventricular and periventricular nuclei, the dorsal border of the ventromedian 
      nucleus, the dorsomedial and arcuate nuclei, and the median eminence. In the 
      latter structure, varicose fibres were mainly distributed in the internal layer 
      and were detected to a lesser extent throughout the external layer, including 
      around the deeper part of the infundibular recess. Most regions of immunoreactive 
      cells and fibres matched perfectly for the two antibodies. However, fibres in the 
      dorsolateral septum, anterior fornix, accumbens nucleus and the lateral bed 
      nucleus of the stria terminalis were only recognised by antibody anti-Kp-10, 
      suggesting that anti-Kp-10 may recognise a wider range of kisspeptin isoforms 
      than anti-Kp-52 or cross-react with molecules other than kisspeptin in rat 
      tissue. Overall, these results illustrate the variety of projection sites of 
      kisspeptin neurones in the rat and suggest that these peptides play a role in 
      different functions.
CI  - (c) 2010 The Authors. Journal of Neuroendocrinology (c) 2010 Blackwell Publishing 
      Ltd.
FAU - Desroziers, E
AU  - Desroziers E
AD  - UMR 6175 INRA/CNRS/Universite de Tours/Haras Nationaux, Nouzilly, France.
FAU - Mikkelsen, J
AU  - Mikkelsen J
FAU - Simonneaux, V
AU  - Simonneaux V
FAU - Keller, M
AU  - Keller M
FAU - Tillet, Y
AU  - Tillet Y
FAU - Caraty, A
AU  - Caraty A
FAU - Franceschini, I
AU  - Franceschini I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Kiss1 protein, rat)
RN  - 0 (Kisspeptins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - *Brain/anatomy & histology/metabolism
MH  - Brain Mapping
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Kisspeptins
MH  - *Nerve Fibers/metabolism/ultrastructure
MH  - *Neurons/cytology/metabolism
MH  - *Proestrus
MH  - Protein Isoforms/*metabolism
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2010/08/03 06:00
MHDA- 2011/01/28 06:00
CRDT- 2010/08/03 06:00
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
AID - JNE2053 [pii]
AID - 10.1111/j.1365-2826.2010.02053.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2010 Oct;22(10):1101-12. doi: 
      10.1111/j.1365-2826.2010.02053.x.