PMID- 20674972
OWN - NLM
STAT- MEDLINE
DCOM- 20101220
LR  - 20211020
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 31
IP  - 9
DP  - 2010 Sep
TI  - Expression of regulatory T cell (Treg) activation markers in endometrial tissues 
      from early and late pregnancy in the feline immunodeficiency virus (FIV)-infected 
      cat.
PG  - 796-802
LID - 10.1016/j.placenta.2010.06.019 [doi]
AB  - Regulatory T cells (Tregs) support pregnancy maintenance by suppressing placental 
      inflammation, while diminished Treg function may accompany reproductive failure. 
      Experimental FIV infection frequently results in vertical transmission and 
      increased pregnancy failure in the cat. The mechanism of reproductive compromise 
      is unknown. We hypothesized that FIV infection alters endometrial Treg population 
      dynamics and function, potentiating vertical transmission and reproductive 
      failure. RNA collected from early and late gestation reproductive tissue and 
      fetuses from FIV infected and control cats was probed for expression of FIV gag 
      and Treg markers CD25, FOXP3, and CTLA4, using real time reverse-transcriptase 
      (RT)-PCR. Frequent placental and fetal infection and reproductive failure were 
      detected at early and late pregnancy. Expression of FOXP3 and CTLA4 was higher in 
      early gestation tissues from control cats. FIV infection significantly reduced 
      expression of FOXP3 and CTLA4 at early, but not late pregnancy. At late 
      pregnancy, CTLA4 was expressed to higher levels in infected tissues. The number 
      of tissues with decreased co-expression of FOXP3 and CTLA4 was significant in 
      infected cats at early pregnancy. No significant changes in CD25 expression 
      occurred between FIV-infected and control animals at early or late pregnancy. 
      Differences in Treg marker expression were not significant between viable and 
      non-viable pregnancies in infected cats. The detection of Treg markers in these 
      feline tissues provides the first evidence of feline endometrial Tregs and 
      suggests that such cells diminish as pregnancy progresses. These cells may be 
      depleted or rendered less functional by viral infection, but understanding their 
      role in pregnancy requires further study.
CI  - Published by Elsevier Ltd.
FAU - Lockett, N N
AU  - Lockett NN
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS 39762, USA.
FAU - Scott, V L
AU  - Scott VL
FAU - Boudreaux, C E
AU  - Boudreaux CE
FAU - Clay, B T
AU  - Clay BT
FAU - Pruett, S B
AU  - Pruett SB
FAU - Ryan, P L
AU  - Ryan PL
FAU - Coats, K S
AU  - Coats KS
LA  - eng
GR  - R15 AI048419/AI/NIAID NIH HHS/United States
GR  - R15 AI048419-02A1/AI/NIAID NIH HHS/United States
GR  - 2R15AI048419-02A1/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100802
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Antigens, CD)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
SB  - IM
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - CTLA-4 Antigen
MH  - Cats
MH  - Feline Acquired Immunodeficiency Syndrome/*immunology
MH  - Female
MH  - Forkhead Transcription Factors/biosynthesis
MH  - Infectious Disease Transmission, Vertical/veterinary
MH  - Interleukin-2 Receptor alpha Subunit/biosynthesis
MH  - Pregnancy/immunology
MH  - Pregnancy Complications, Infectious/*veterinary
MH  - T-Lymphocytes, Regulatory/*immunology
PMC - PMC2933968
MID - NIHMS223692
EDAT- 2010/08/03 06:00
MHDA- 2010/12/21 06:00
PMCR- 2011/09/01
CRDT- 2010/08/03 06:00
PHST- 2010/05/18 00:00 [received]
PHST- 2010/06/25 00:00 [revised]
PHST- 2010/06/29 00:00 [accepted]
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2010/12/21 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - S0143-4004(10)00249-3 [pii]
AID - 10.1016/j.placenta.2010.06.019 [doi]
PST - ppublish
SO  - Placenta. 2010 Sep;31(9):796-802. doi: 10.1016/j.placenta.2010.06.019. Epub 2010 
      Aug 2.