PMID- 20674997
OWN - NLM
STAT- MEDLINE
DCOM- 20120813
LR  - 20111031
IS  - 1874-1754 (Electronic)
IS  - 0167-5273 (Linking)
VI  - 152
IP  - 3
DP  - 2011 Nov 3
TI  - Mesenchymal stem cell injection ameliorates the inducibility of ventricular 
      arrhythmias after myocardial infarction in rats.
PG  - 314-20
LID - 10.1016/j.ijcard.2010.07.025 [doi]
AB  - BACKGROUND: Mesenchymal stem cell transplantation is a promising new therapy to 
      improve cardiac function after myocardial infarction (MI). The 
      electrophysiological consequences of MSC implantation has not been systematically 
      studied. METHODS: We investigated the electrophysiological and arrhythmogenic 
      effects of mesenchymal stem cells (MSCs) therapy in experimental infarction 
      model. Rats were subjected to MI operation by LAD ligation and randomly allocated 
      to receive intramyocardially injection PBS (MI-PBS) or 5 x 10(5) EGFP labeled 
      MSCs (MI-MSCs). Electrophysiological study, histological examination, and western 
      blotting were performed 2 weeks after cell transplantation. RESULTS: Programmed 
      electrical stimulation (PES) showed a significant reduced inducible ventricular 
      tachycardias (VTs), raised ventricular fibrillation threshold (VFT) and prolonged 
      ventricular effective refractory period (VERP) in MSC-treated rats compared to 
      PBS-treated animals. MSC implantation led to markedly longer action potential 
      duration (APD) and shorter activation time (AT) in infarcted border zone (IBZ) of 
      left ventricular epicardium compared with PBS-treated hearts. Histological study 
      revealed that fibrotic area and collagen deposition in infarcted region were 
      significantly lower in MI-MSC group than in MI-PBS group. Abnormal alterations of 
      Connexin 43 including reduction and lateralization were significantly attenuated 
      by MSC treatment. CONCLUSIONS: This study provide strong evidence that MSC 
      implantation ameliorates interstitial fibrosis and the remodeling of gap 
      junction, attenuates focal heterogeneity of reporlarization and conduction and 
      reduces vulnerability to VTs. The results suggest that MSC transplantation might 
      emerge as a new preventive strategy against VAs besides improving cardiac 
      performance in ischemic heart disease.
CI  - Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Wang, Deguo
AU  - Wang D
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, PR China.
FAU - Zhang, Fengxiang
AU  - Zhang F
FAU - Shen, Wenzhi
AU  - Shen W
FAU - Chen, Minglong
AU  - Chen M
FAU - Yang, Bing
AU  - Yang B
FAU - Zhang, Yuzhen
AU  - Zhang Y
FAU - Cao, Kejiang
AU  - Cao K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100802
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Female
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Myocardial Infarction/physiopathology/*surgery
MH  - Random Allocation
MH  - Rats
MH  - Tachycardia, Ventricular/physiopathology/*prevention & control/surgery
EDAT- 2010/08/03 06:00
MHDA- 2012/08/14 06:00
CRDT- 2010/08/03 06:00
PHST- 2010/04/06 00:00 [received]
PHST- 2010/07/04 00:00 [accepted]
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2012/08/14 06:00 [medline]
AID - S0167-5273(10)00552-8 [pii]
AID - 10.1016/j.ijcard.2010.07.025 [doi]
PST - ppublish
SO  - Int J Cardiol. 2011 Nov 3;152(3):314-20. doi: 10.1016/j.ijcard.2010.07.025. Epub 
      2010 Aug 2.